Rohani et al show that converting human pluripotent stem cells (hPSCs) to the naïve state enhances hPSC manufacturing. Further, through transcriptomics, the expression of epigenetic regulators, metabolomics, and cell-surface protein marker analyses, they indicate a potentially important role for the stirred bioreactor’s mechanical environment in maintaining naïve-like pluripotency.
- Leili Rohani
- Breanna S. Borys
- Derrick E. Rancourt
