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Showing 1–10 of 10 results
Advanced filters: Author: Hideharu Mikami Clear advanced filters

  • High throughput imaging flow cytometry suffers from trade-offs between throughput, sensitivity and spatial resolution. Here the authors introduce a method to virtually freeze cells in the image acquisition window to enable 1000 times longer signal integration time and improve signal-to-noise ratio.

    • Hideharu Mikami
    • Makoto Kawaguchi
    • Keisuke Goda
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-11

  • Flow cytometry is often hindered by undesired fluctuations in fluorescence intensity. Here, the authors propose high-throughput fluorescence lifetime imaging flow cytometry, which enables imaging at a rate of over 10,000 cells per second and, therefore, enhances the capabilities of cellular analysis.

    • Hiroshi Kanno
    • Kotaro Hiramatsu
    • Keisuke Goda
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-15

  • Most current cell sorting methods are based on fluorescence detection with no imaging capability. Here the authors generate and use Raman image-activated cell sorting with a throughput of around 100 events per second, providing molecular images with no need for labeling.

    • Nao Nitta
    • Takanori Iino
    • Keisuke Goda
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-16

  • A protocol for the design, construction, and operation of an intelligent image-activated cell sorting (iIACS) machine that performs real-time image-based sorting of single cells from heterogeneous populations with high throughput and intelligence.

    • Akihiro Isozaki
    • Hideharu Mikami
    • Keisuke Goda
    Protocols
    Nature Protocols
    Volume: 14, P: 2370-2415

  • A total of 1074 patients with a history of ischemic stroke enrolled in The Recurrent Stroke Prevention Clinical Outcome (RESPECT) Study were assigned to intensive blood pressure (BP) control group (blood pressure < 120/80 mmHg) or standard blood pressure control group (blood pressure < 140/90 mmHg) and were followed up for a mean of 3.9 years. Seventy-eight first recurrent strokes occurred, including 70 ischemic stroke and 8 intracerebral hemorrhage. Intensive treatment did not change annual risk of ischemic stroke (1.74% vs. 1.75%, P = 0.999), but markedly reduced risk of hemorrhagic stroke (0.00% vs. 0.39%, P = 0.005). Beneficial effect of intensive BP control were observed for risk of hemorrhagic stroke in the patients with a history of ischemic stroke.

    • Kazuo Kitagawa
    • Hisatomi Arima
    • Seigo Nakada
    Research
    Hypertension Research
    Volume: 45, P: 591-601

  • This protocol describes how to build an optical time-stretch microscope and a cell-focusing microfluidic device for high-throughput (>10,000 cells per s) single-cell imaging by optofluidic time-stretch microscopy.

    • Cheng Lei
    • Hirofumi Kobayashi
    • Keisuke Goda
    Protocols
    Nature Protocols
    Volume: 13, P: 1603-1631