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Showing 1–11 of 11 results
Advanced filters: Author: Holger Fleckenstein Clear advanced filters

  • The new European X-Ray Free-Electron Laser (EuXFEL) is the first XFEL that generates X-ray pulses with a megahertz inter-pulse spacing. Here the authors demonstrate that high-quality and damage-free protein structures can be obtained with the currently available 1.1 MHz repetition rate pulses using lysozyme as a test case and furthermore present a β-lactamase structure.

    • Max O. Wiedorn
    • Dominik Oberthür
    • Anton Barty
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-11

  • Lipidic sponge phase crystallization yields membrane protein microcrystals that can be injected into an X-ray free electron laser beam, yielding diffraction patterns that can be processed to recover the crystal structure.

    • Linda C Johansson
    • David Arnlund
    • Richard Neutze
    Research
    Nature Methods
    Volume: 9, P: 263-265

  • Researchers describe a mechanism capable of compressing fast and intense X-ray pulses through the rapid loss of crystalline periodicity. It is hoped that this concept, combined with X-ray free-electron laser technology, will allow scientists to obtain structural information at atomic resolutions.

    • Anton Barty
    • Carl Caleman
    • Henry N. Chapman
    Research
    Nature Photonics
    Volume: 6, P: 35-40

  • Due to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immiscible oil and demonstrate that with this device an approx. 60% reduction in sample waste was achieved for data collection of 3-deoxy-D-manno-octulosonate 8-phosphate synthase crystals at the EuXFEL.

    • Austin Echelmeier
    • Jorvani Cruz Villarreal
    • Alexandra Ros
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-10

  • XFEL radiation is providing new opportunities for probing biological systems. Here the authors perform nanoscale x-ray imaging of microtubules with helical symmetry, by using imaging sorting and reconstruction techniques.

    • Gisela Brändén
    • Greger Hammarin
    • Richard Neutze
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-9

  • Femtosecond X-ray pulses were used to obtain diffraction data on photosystem II, revealing conformational changes as the complex transitions from the dark S1 state to the double-pumped S3 state; the time-resolved serial femtosecond crystallography technique enables structural determination of protein conformations that are highly prone to traditional radiation damage.

    • Christopher Kupitz
    • Shibom Basu
    • Petra Fromme
    Research
    Nature
    Volume: 513, P: 261-265

  • High-resolution, low-dose images of biological objects were obtained in a new method using photons of 60 keV. The dose was thousands of times lower than conventional methods, overcoming the previous limitations set by radiation damage to the sample.

    • Tang Li
    • J. Lukas Dresselhaus
    • Saša Bajt
    ResearchOpen Access
    Light: Science & Applications
    Volume: 12, P: 1-12