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Showing 1–11 of 11 results
Advanced filters: Author: Holger Puchta Clear advanced filters
  • How double strand breaks (DSBs) are repaired within the plant 45S rDNA repeats is unclear. Here, the authors show that Cas9-mediated DSBs in 45S rDNA are mainly repaired by cNHEJ and describe CRISPR-Kill as a tool for organ-specific cell elimination by targeting functional repetitive DNA in Arabidopsis.

    • Angelina Schindele
    • Fabienne Gehrke
    • Holger Puchta
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-10
  • The heterochromatic knob (hk4S) on Arabidopsis chromosome 4 prevents the recombination between accessions with and without hk4S. Here, via egg-cell specific expression of the Cas9 nuclease, the authors demonstrate targeted reversal of the 1.1 Mb long hk4S-inversion in Col-0 and restore the crossovers with Ler-1.

    • Carla Schmidt
    • Paul Fransz
    • Holger Puchta
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-8
  • The inclusion of retrotransposon long terminal repeats — and of other repeated sequences — enhances transfer DNA copy numbers in plant cells during transformation. Gene editing and homologous recombination-mediated gene targeting can therefore be improved by these means: however, the mechanism remains a mystery.

    • Holger Puchta
    News & Views
    Nature Plants
    Volume: 9, P: 1377-1378
  • A direct link between DNA repair and heat tolerance has been revealed in Arabidopsis thaliana.

    • Annika Dorn
    • Holger Puchta
    News & Views
    Nature Plants
    Volume: 6, P: 1398-1399
  • Future genome editing in plants will mimic the natural evolutionary processes that shape genomes. It will be used to reshape plant genomes in a manner that could have happened naturally, but more precisely and more rapidly.

    • Larry Gilbertson
    • Holger Puchta
    • R. Keith Slotkin
    Reviews
    Nature Plants
    Volume: 11, P: 680-685
  • Manipulation of genetic exchange is an important objective of plant breeders. Using chromosome engineering to invert a 17.1 Mb fragment on chromosome 2 in Arabidopsis thaliana, meiotic recombination could be suppressed in nearly the entire chromosome.

    • Michelle Rönspies
    • Carla Schmidt
    • Holger Puchta
    Research
    Nature Plants
    Volume: 8, P: 1153-1159
  • Clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein (Cas) technology was used to induce heritable reciprocal chromosomal translocations in the Mb range in Arabidopsis, providing new possibilities to modify chromosomes and break linkage drag in plant breeding.

    • Natalja Beying
    • Carla Schmidt
    • Holger Puchta
    Research
    Nature Plants
    Volume: 6, P: 638-645
  • RecQ helicases are enzymes that play a central role in maintaining genome stability in the DNA repair cascade. Klaue et al. show that RecQ2 and RecQ3 from Arabidopsis thalianaprocess DNA by, respectively, unwinding and rewinding forked DNA substrates, using a frequent strand switching mechanism.

    • Daniel Klaue
    • Daniela Kobbe
    • Ralf Seidel
    ResearchOpen Access
    Nature Communications
    Volume: 4, P: 1-9
  • This chromosome-engineering protocol generates heritable chromosomal rearrangements in A. thaliana; by combining SaCas9 with an egg-cell-specific promoter to facilitate heritable mutations, chromosomal rearrangements can be made and homozygous lines can be established.

    • Michelle Rönspies
    • Patrick Schindele
    • Holger Puchta
    Protocols
    Nature Protocols
    Volume: 17, P: 1332-1358