Nature Search

Modular detergents tailor the purification and structural analysis of membrane proteins including G-protein coupled receptors

Detergents are indispensable reagents in membrane protein structural biology. Here, L. H. Urner and co-workers introduce oligoglycerol detergents (OGDs) and use native mass spectrometry to show how interactions of membrane proteins with native membrane lipids can be preserved during purification.

Leonhard H. Urner
Idlir Liko
Kevin Pagel
ResearchOpen Access28 Jan 2020
Nature Communications

Volume: 11, P: 1-10
Human glycogenins maintain glucose homeostasis by regulating glycogen metabolism

Glycogenin initiates glycogen synthesis, but its two human isoforms may do more than expected. Here, Weng et al. reveal that GYG2, despite low enzyme activity, drives glycogen particle assembly, uncovering a new layer of metabolic control.

Tzu-Han Weng
Yu-Chung Pien
Su-Yi Tsai
ResearchOpen Access16 Jul 2025
Nature Communications

Volume: 16, P: 1-21
Ligand-induced conformational changes in the β1-adrenergic receptor revealed by hydrogen-deuterium exchange mass spectrometry

GPCRs are vital drug targets. Here, authors use HDX-MS to gain insight into structural dynamics of β1AR upon ligand binding, revealing agonist-induced destabilisation and antagonist stabilisation of ICL1.

Joanna Toporowska
Parth Kapoor
Argyris Politis
ResearchOpen Access18 Oct 2024
Nature Communications

Volume: 15, P: 1-14
Mass spectrometry captures biased signalling and allosteric modulation of a G-protein-coupled receptor

Native mass spectrometry has been used to interrogate both biased signalling and allosteric modulation of the β₁-adrenergic receptor. Simultaneously capturing the effects of ligand binding and receptor coupling to different G proteins has enabled the relative importance of specific interactions to be investigated.

Hsin-Yung Yen
Idlir Liko
Carol V. Robinson
ResearchOpen Access10 Nov 2022
Nature Chemistry

Volume: 14, P: 1375-1382
Structures of the archaerhodopsin-3 transporter reveal that disordering of internal water networks underpins receptor sensitization

Archaerhodopsin-3 (AR3) mutants are commonly used in optogenetics for neuron silencing and membrane voltage sensing. High-resolution crystal structures show that desensitization of the AR3 photoreceptor occurs when internal hydrogen-bonded water networks are modified in response to changes in chromophore isomerization.

Juan F. Bada Juarez
Peter J. Judge
Anthony Watts
ResearchOpen Access27 Jan 2021
Nature Communications

Volume: 12, P: 1-10
Structural insights into the lipid and ligand regulation of serotonin receptors

Cryo-electron microscopy structures of three different serotonin receptors in complex with serotonin and other agonists provide insights into the role of lipids in regulating these receptors and the structural basis of ligand recognition.

Peiyu Xu
Sijie Huang
H. Eric Xu
Research24 Mar 2021
Nature

Volume: 592, P: 469-473
Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics

Measuring phosphorylation stoichiometry on a proteomic scale remains a challenge. Tsai et al. develop a technique to measure the basal level of phosphorylation stoichiometry in a single human phosphoproteome and identify molecular changes associated with gefitinib resistance in lung cancer cells.

Chia-Feng Tsai
Yi-Ting Wang
Yu-Ju Chen
ResearchOpen Access27 Mar 2015
Nature Communications

Volume: 6, P: 1-8
Combining native and ‘omics’ mass spectrometry to identify endogenous ligands bound to membrane proteins

‘Nativeomics’ enables identification of ligands bound to membrane proteins through detection of intact protein–ligand assemblies followed by dissociation and identification of individual ligands within the same mass spectrometry experiment.

Joseph Gault
Idlir Liko
Carol V. Robinson
Research04 May 2020
Nature Methods

Volume: 17, P: 505-508
PtdIns(4,5)P₂ stabilizes active states of GPCRs and enhances selectivity of G-protein coupling

Mass spectrometry-based assays are used to reveal specificity and structural determinants of lipid binding to class A G-protein-coupled receptors, and the effects of specific lipids on receptor coupling to G proteins.

Hsin-Yung Yen
Kin Kuan Hoi
Carol V. Robinson
Research11 Jul 2018
Nature

Volume: 559, P: 423-427
Phosphoproteomics Reveals HMGA1, a CK2 Substrate, as a Drug-Resistant Target in Non-Small Cell Lung Cancer

Yi-Ting Wang
Szu-Hua Pan
Yu-Ju Chen
ResearchOpen Access14 Mar 2017
Scientific Reports

Volume: 7, P: 1-14

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