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Showing 1–4 of 4 results
Advanced filters: Author: Hwangbeom Kim Clear advanced filters
  • One of the biggest bottlenecks in large-scale DNA synthesis is the retrieval of target clonal DNA from high-density sequencing platforms. Here, the authors present a method called ‘Sniper Cloning’ that allows for precise mapping of target clone features and rapid retrieval of targets for full utilization of DNA clones.

    • Howon Lee
    • Hyoki Kim
    • Sunghoon Kwon
    ResearchOpen Access
    Nature Communications
    Volume: 6, P: 1-7
  • The use of marker coselection in combination with multiplex automated genome engineering (MAGE) is reported to improve the efficiency of engineered changes in bacterial genomes. The authors use the method to insert twelve 20-base-pair T7 promoters to control indirubin and indigo production.

    • Harris H Wang
    • Hwangbeom Kim
    • George M Church
    Research
    Nature Methods
    Volume: 9, P: 591-593
  • Protein-protein interactions (PPIs) are crucial for biological functions and have applications ranging from drug design to synthetic cell circuits. Here the authors develop an assay and computational methods to identify more orthogonal coiled-coil pairs, critical for biological processes and drug design.

    • W. Clifford Boldridge
    • Ajasja Ljubetič
    • Sriram Kosuri
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-11