Dieterich et al. describe a methodology to label all newly synthesized neuronal proteins in situ. This method, which they name FUNCAT, relies on the inclusion of noncanonical amino acids and selective fluorescent labeling via click chemistry. The authors show that this system is amenable to dual pulse-chase experiments and dynamic tracking of newly synthesized proteins.
- Daniela C Dieterich
- Jennifer J L Hodas
- Erin M Schuman
