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RoCK and ROI: single-cell transcriptomics with multiplexed enrichment of selected transcripts and region-specific sequencing

Current single-cell RNA sequencing methods struggle to comprehensively profile transcriptomes, with many lowly expressed transcripts remaining undetected. Here authors present a workflow for enhancing the detection of both transcripts and regions of interest in combination with a standard transcriptome profile.

Giulia Moro
Izaskun Mallona
Konrad Basler
ResearchOpen Access10 Dec 2025
Nature Communications

Volume: 16, P: 1-22
Targeted CRISPR-Cas9 screening identifies core transcription factors controlling murine haemato-endothelial fate commitment

During development, blood is generated in the yolk sac by differentiation of haemato-endothelial mesoderm into haematopoietic progenitors. Using CRISPR-Cas9 screens, the authors identify Etv2, Smad1, Ldb1, Six4 and Zbtb7b as regulators of haemato-endothelial mesoderm commitment. Lack of these regulators gave rise to mesodermal subsets with a defined lineage differentiation bias.

Michael Teske
Tobias Wertheimer
Nina Schmolka
ResearchOpen Access13 Dec 2025
Nature Communications

Volume: 16, P: 1-15
A model of human neural networks reveals NPTX2 pathology in ALS and FTLD

A neural stem cell culture system derived from induced pluripotent stem cells forms a network of synaptically connected and electrophysiologically active neurons that were used as a model system to identify a mechanism of TDP-43-induced neurodegeneration.

Marian Hruska-Plochan
Vera I. Wiersma
Magdalini Polymenidou
ResearchOpen Access14 Feb 2024
Nature

Volume: 626, P: 1073-1083
Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging

Neurons are generated throughout life in the mammalian hippocampus. Bottes et al. used intravital imaging and comparative single-cell transcriptomics to identify long-term self-renewing neural stem cells in the adult mouse hippocampus.

Sara Bottes
Baptiste N. Jaeger
Sebastian Jessberger
Research21 Dec 2020
Nature Neuroscience

Volume: 24, P: 225-233

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RoCK and ROI: single-cell transcriptomics with multiplexed enrichment of selected transcripts and region-specific sequencing

Targeted CRISPR-Cas9 screening identifies core transcription factors controlling murine haemato-endothelial fate commitment

A model of human neural networks reveals NPTX2 pathology in ALS and FTLD

Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging

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