The authors developed two subcellular optogenetic tools, pHoenix and lyso-pHoenix, that allow light-driven acidification of synaptic vesicles and lysosomes, respectively. pHoenix was used to control the degree of neurotransmitter uptake into synaptic vesicles, revealing that exocytosis of partially filled vesicles is less efficient than the release of completely filled vesicles.
- Benjamin R Rost
- Franziska Schneider
- Christian Rosenmund
