Whether 5-formylcytosine (f5C) is an epitranscriptomic mark is controversial. This study reveals pitfalls in previous f5C-detection methods. Instead, they developed FIBo-seq and find no f5C in mammalian RNA beyond the known mitochondrial tRNAMet.
- Jasmin A. Dehnen
- Alexander V. Gopanenko
- Christof Niehrs