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Showing 1–4 of 4 results

Advanced filters: Author: Jean Ollion Clear advanced filters

Real-time visualization of mutations and their fitness effects in single bacteria

This protocol uses a microfluidic device and microscopy to detect mutations occurring as a consequence of DNA replication and assess their effects on fitness in Escherichia coli single cells.

Lydia Robert
Jean Ollion
Marina Elez
Protocols25 Sept 2019
Nature Protocols

Volume: 14, P: 3126-3143
Real-time monitoring of replication errors’ fate reveals the origin and dynamics of spontaneous mutations

An interdisciplinary approach following replication errors in Escherichia coli unveils that many spontaneous mutations originate from inefficient repair, and that repair capacity is variable between single cells within a bacterial population.

Chiara Enrico Bena
Jean Ollion
Marina Elez
ResearchOpen Access27 Mar 2024
Nature Communications

Volume: 15, P: 1-11
High-throughput detection and tracking of cells and intracellular spots in mother machine experiments

BACMMAN software is used to automate image analysis of high-throughput 2D or 3D time-series images from experiments using the ‘mother machine’, a microfluidic device that allows growth and division of single bacterial cells to be followed.

Jean Ollion
Marina Elez
Lydia Robert
Protocols25 Sept 2019
Nature Protocols

Volume: 14, P: 3144-3161
Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity

A dual illumination method reduces photobleaching for green and yellow fluorescent proteins.

Lucie Ludvikova
Emma Simon
Agathe Espagne
ResearchOpen Access03 Aug 2023
Nature Biotechnology

Volume: 42, P: 872-876