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Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

It can be difficult to find rare transcripts when sequencing a transcriptome. Here the authors show adaptive sampling on direct RNA runs to increase the likelihood of finding less frequent ones while selectively ejecting the higher-abundance transcripts.

Jiaxu Wang
Lin Yang
Yue Wan
ResearchOpen Access11 Jan 2024
Nature Communications

Volume: 15, P: 1-10
RNA structure profiling at single-cell resolution reveals new determinants of cell identity

Single-cell structure probing of RNA transcripts enables simultaneous determination of transcript secondary structure and abundance in single cells, allowing new insights into RNA structural heterogeneity within and among cells.

Jiaxu Wang
Yu Zhang
Yue Wan
ResearchOpen Access04 Jan 2024
Nature Methods

Volume: 21, P: 411-422

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Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

RNA structure profiling at single-cell resolution reveals new determinants of cell identity

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