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Spatial biology using single-cell mass spectrometry imaging and integrated microscopy

Potthoff and colleagues present a MALDI-MSI-based method that integrates in-source brightfield and fluorescence microscopy, which allows for spatially-resolved analysis of lipids and metabolites at the (sub)cellular level.

Alexander Potthoff
Jan Schwenzfeier
Jens Soltwisch
ResearchOpen Access15 Oct 2025
Nature Communications

Volume: 16, P: 1-16
Subcellular mass spectrometry imaging of lipids and nucleotides using transmission geometry ambient laser desorption and plasma ionisation

Young and colleagues describe an atmospheric pressure transmission-geometry MALDI approach equipped with plasma ionisation, which allows for subcellular mass spectrometry imaging of lipids and nucleotides in cells and tissues.

Reuben S. E. Young
Ann-Katrin Piper
Shane R. Ellis
ResearchOpen Access15 Oct 2025
Nature Communications

Volume: 16, P: 1-13
msiFlow: automated workflows for reproducible and scalable multimodal mass spectrometry imaging and microscopy data analysis

This paper introduces msiFlow, an open-source software for scalable and reproducible end-to-end multimodal image analysis. Using msiFlow, the authors explore the molecular heterogeneity of leukocytes in infected tissues.

Philippa Spangenberg
Sebastian Bessler
Daniel R. Engel
ResearchOpen Access27 Jan 2025
Nature Communications

Volume: 16, P: 1-13
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling

A correlative imaging approach in which matrix-assisted laser desorption ionization mass spectrometry imaging is followed by fluorescence in situ hybridization (metaFISH) for the spatial metabolomics identification of host–microbiome interactions, at single-cell resolution, via nucleic acid probes.

Patric Bourceau
Benedikt Geier
Manuel Liebeke
Protocols06 Sept 2023
Nature Protocols

Volume: 18, P: 3050-3079
New insights into mechanisms of material ejection in MALDI mass spectrometry for a wide range of spot sizes

Marcel Niehaus
Jens Soltwisch
ResearchOpen Access17 May 2018
Scientific Reports

Volume: 8, P: 1-10

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Spatial biology using single-cell mass spectrometry imaging and integrated microscopy

Subcellular mass spectrometry imaging of lipids and nucleotides using transmission geometry ambient laser desorption and plasma ionisation

msiFlow: automated workflows for reproducible and scalable multimodal mass spectrometry imaging and microscopy data analysis

Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling

New insights into mechanisms of material ejection in MALDI mass spectrometry for a wide range of spot sizes

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