Efforts to study ubiquitination have been hampered by the large size of ubiquitin relative to other post-translational modifications. Xu et al. use a monoclonal antibody specific for the adduct left after proteolysis of ubiquitinated proteins to analyze the differential regulation of ubiquitination at distinct sites within the same proteins.
- Guoqiang Xu
- Jeremy S Paige
- Samie R Jaffrey
