We describe generation of neuroblastoma cell lines using conditional reprogramming (CR)—new cell culture method that allows indefinite propagation of cells in an undifferentiated state. We optimized CR growth conditions for neuroblastoma tissue and grew mouse neuroblastoma cells in vitro. These cells demonstrate a unique phenotype, are positive for neuroblastoma markers, and can be passaged and biobanked for continued studies.
- Ewa Krawczyk
- Sung-Hyeok Hong
- Joanna Kitlinska
