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Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy

The authors introduce fluorescence lifetime microscopy using a novel SPAD array detector in a single-objective light-sheet microscope. This imaging system achieves 10–100-fold improvement in imaging speed compared to a confocal fluorescence lifetime imaging microscope.

Valentin Dunsing-Eichenauer
Johan Hummert
Pierre-François Lenne
ResearchOpen Access29 Nov 2025
Communications Biology

Volume: 8, P: 1-11
ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting

Protein-tag degree of labeling (ProDOL) is a versatile reference-based approach for experimentally determining the degree of target labeling for improved protein counting and quantification and for optimizing labeling protocols in fixed and live cells.

Stanimir Asenov Tashev
Jonas Euchner
Dirk-Peter Herten
ResearchOpen Access08 Aug 2024
Nature Methods

Volume: 21, P: 1708-1715
Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching

It is currently debated how to reliably distinguish liquid–liquid phase separation (LLPS) from other mechanisms. Here the authors report model-free calibrated half-FRAP (MOCHA-FRAP) to probe the barrier at the condensate interface that is responsible for preferential internal mixing in LLPS.

Fernando Muzzopappa
Johan Hummert
Fabian Erdel
ResearchOpen Access16 Dec 2022
Nature Communications

Volume: 13, P: 1-15
Divalent nanobodies to platelet CLEC-2 can serve as agonists or antagonists

Fluorescence correlation spectroscopy on crosslinked nanobodies tested on C-type lectin-like receptor 2 (CLEC-2) show different outcomes for divalent and tetravalent nanobody ligands on CLEC-2 clustering, also dependent on CLEC-2 expression levels.

Joanne C. Clark
Eleyna M. Martin
Steve P. Watson
ResearchOpen Access07 Apr 2023
Communications Biology

Volume: 6, P: 1-17

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Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy

ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting

Detecting and quantifying liquid–liquid phase separation in living cells by model-free calibrated half-bleaching

Divalent nanobodies to platelet CLEC-2 can serve as agonists or antagonists

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