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Cryo-EM structure and B-factor refinement with ensemble representation

Cryo-EM is the go-to method for visualizing large, flexible biomolecules. Here, authors introduce a new Gaussian mixture modelling method for cryo-EM modelling tasks, including refinement, composite map generation and ensemble representation.

Joseph G. Beton
Thomas Mulvaney
Maya Topf
ResearchOpen Access10 Jan 2024
Nature Communications

Volume: 15, P: 1-13
Base-pair resolution analysis of the effect of supercoiling on DNA flexibility and major groove recognition by triplex-forming oligonucleotides

In cells, DNA is arranged into topologically-constrained (supercoiled) structures, but how this supercoiling affects the detailed double-helical structure of DNA remains unclear. Here authors use atomic force microscopy and atomistic molecular dynamics simulations, to resolve structures of negatively-supercoiled DNA minicircles at base-pair resolution.

Alice L. B. Pyne
Agnes Noy
Sarah A. Harris
ResearchOpen Access16 Feb 2021
Nature Communications

Volume: 12, P: 1-12
Stepwise recruitment of chaperone Hsc70 by DNAJB1 produces ordered arrays primed for bursts of amyloid fibril disassembly

Cryo-EM and tomography of the Hsp70 chaperone system disaggregating amyloid fibrils show that the 2 step activation of the class B J-domain protein DNAJB1 is required for the formation of ordered arrays of chaperones that prime fibril disassembly.

Jim Monistrol
Joseph G. Beton
Helen R. Saibil
ResearchOpen Access30 Mar 2025
Communications Biology

Volume: 8, P: 1-15

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Cryo-EM structure and B-factor refinement with ensemble representation

Base-pair resolution analysis of the effect of supercoiling on DNA flexibility and major groove recognition by triplex-forming oligonucleotides

Stepwise recruitment of chaperone Hsc70 by DNAJB1 produces ordered arrays primed for bursts of amyloid fibril disassembly

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