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Showing 1–4 of 4 results
Advanced filters: Author: Joyce Woodhouse Clear advanced filters

  • Providing detailed structural descriptions of the ultrafast photochemical events that occur in light-sensitive proteins is key to their understanding. Now, excited-state structures in the reversibly switchable fluorescent protein rsEGFP2 have been solved by time-resolved crystallography using an X-ray laser. These structures enabled the design of a mutant with improved photoswitching quantum yields.

    • Nicolas Coquelle
    • Michel Sliwa
    • Martin Weik
    Research
    Nature Chemistry
    Volume: 10, P: 31-37

  • rsEGFP2 is a reversibly photoswitchable fluorescent protein used in super-resolution light microscopy. Here the authors present the structure of an rsEGFP2 ground-state intermediate after excited state-decay that was obtained by nanosecond time-resolved serial femtosecond crystallography at an X-ray free electron laser, and time-resolved absorption spectroscopy measurements complement their structural analysis.

    • Joyce Woodhouse
    • Gabriela Nass Kovacs
    • Martin Weik
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-11

  • X-ray crystallography is the main method for protein structure determination. Here the authors combine solid-state NMR measurements and molecular dynamics simulations and show that crystal packing alters the thermodynamics and kinetics of local conformational exchange as well as overall rocking motion of protein molecules in the crystal lattice.

    • Vilius Kurauskas
    • Sergei A. Izmailov
    • Paul Schanda
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-12

  • Heyes et al. report that the conformational changes of a full-length Synechocystis phytochrome from the red- to the far-red-absorbing states extend to the seconds timescale. Interestingly, while the rapid structural changes are independent of the output domain the slower changes are not, providing useful insights on photosensory-region dynamics.

    • Derren J. Heyes
    • Samantha J. O. Hardman
    • Giorgio Schirò
    ResearchOpen Access
    Communications Biology
    Volume: 2, P: 1-8