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Showing 1–6 of 6 results
Advanced filters: Author: Julius Fredens Clear advanced filters

  • High-fidelity convergent total synthesis is used to produce Escherichia coli with a 61-codon synthetic genome that uses 59 codons to encode all of the canonical amino acids.

    • Julius Fredens
    • Kaihang Wang
    • Jason W. Chin
    Research
    Nature
    Volume: 569, P: 514-518

  • BAC stepwise insertion synthesis (BASIS) can be used to build synthetic genomes for diverse organisms, and continuous genome synthesis (CGS) enables the rapid synthesis of entire Escherichia coli genomes from functional designs.

    • Jérôme F. Zürcher
    • Askar A. Kleefeldt
    • Jason W. Chin
    Research
    Nature
    Volume: 619, P: 555-562

  • REXER, a new method that allows long sections of DNA to be inserted or replaced in the genome of the bacterium Escherichia coli, is used to investigate codon replacement schemes for the generation of synthetic genomes.

    • Kaihang Wang
    • Julius Fredens
    • Jason W. Chin
    Research
    Nature
    Volume: 539, P: 59-64

  • A method for performing quantitative proteomics experiments in Caenorhabditis elegans using stable-isotope labeling of lysine is described. The method can be coupled with RNA interference to examine global effects on the proteome. Also in this issue, Larance et al. describe a very similar method.

    • Julius Fredens
    • Kasper Engholm-Keller
    • Nils J Færgeman
    Research
    Nature Methods
    Volume: 8, P: 845-847

  • This protocol describes a recombination-based approach for accurate replacement of genomic DNA with large synthetic DNA fragments in E. coli (REXER). Iterative repetition of REXER (GENESIS) combined with genome assembly methods enables the creation of custom synthetic E. coli genomes.

    • Wesley E. Robertson
    • Louise F. H. Funke
    • Jason W. Chin
    Protocols
    Nature Protocols
    Volume: 16, P: 2345-2380