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Showing 1–6 of 6 results
Advanced filters: Author: Kam Hon Hoi Clear advanced filters

  • Isolation of antigen-specific antibodies or antibody fragments, whether through B-cell immortalization or recombinant libraries, generally requires laborious screening. Reddy et al. circumvent this step using high-throughput sequencing of plasma cells and bioinformatic analysis of the variable-gene repertoire.

    • Sai T Reddy
    • Xin Ge
    • George Georgiou
    Research
    Nature Biotechnology
    Volume: 28, P: 965-969

  • Leonard Goldstein et al. use high-throughput single-cell B-cell receptor sequencing on thousands of individual B cells from rat, mouse, and human repertoires. They obtained paired full-length heavy- and light-chain variable regions, and show that this approach is a powerful tool for antibody discovery.

    • Leonard D. Goldstein
    • Ying-Jiun J. Chen
    • Somasekar Seshagiri
    ResearchOpen Access
    Communications Biology
    Volume: 2, P: 1-10

  • Sankar et al. investigate the junctional length biases (determining antibody binding potential) as a function of germline gene usage in antibody repertoires. They show that CDR H3 and junction length are biased by VH, VL, and JH germline segment usage and these biases are apparent in both naive and antigen-experienced repertoires but not in non-productive repertoires.

    • Kannan Sankar
    • Kam Hon Hoi
    • Isidro Hötzel
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-10

  • In an inter-laboratory study, the authors compare the accuracy and performance of three optical density calibration protocols (colloidal silica, serial dilution of silica microspheres, and colony-forming unit (CFU) assay). They demonstrate that serial dilution of silica microspheres is the best of these tested protocols, allowing precise and robust calibration that is easily assessed for quality control and can also evaluate the effective linear range of an instrument.

    • Jacob Beal
    • Natalie G. Farny
    • Jiajie Zhou
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-29