This review highlights recent advances in engineering artificial enzyme condensates in vitro using charged polymers. Based on our recent findings, we describe strategies for designing condensates through interactions between polymers and enzymes or coenzymes. We then summarize enzyme activation mechanisms triggered by enzyme condensates, including size-dependent effects and conformational changes in enzymes. We also discuss potential applications and future directions, including multienzyme systems, integration with solid surfaces, and combination with rational enzyme design.
- Tomoto Ura
- Toya Yoshida
- Kentaro Shiraki
