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Showing 1–21 of 21 results
Advanced filters: Author: Knut Woltjen Clear advanced filters

  • Precision editing with CRISPR-Cas9 often suffers from poor efficiency. Here the authors identify culture conditions and small molecules that synergize to promote homology-directed repair (HDR) in induced pluripotent stem (iPS) cells.

    • Thomas L. Maurissen
    • Knut Woltjen
    ResearchOpen Access
    Nature Communications
    Volume: 11, P: 1-14

  • Through non-cell-autonomous functions, senescent cells can induce secondary senescence in bystander cells. Sogabe et al. generate mouse models of MEK1- or MKK6-induced senescence and use double labeling to map primary and secondary senescence in vivo using single-cell and spatial analysis.

    • Yuko Sogabe
    • Hirofumi Shibata
    • Takuya Yamamoto
    Research
    Nature Aging
    Volume: 5, P: 1568-1588

  • This paper presents a technique to reprogram mouse and human fibroblasts to induce pluripotency. The authors show that the transgene can be removed once reprogramming has been achieved with a piggyBac transposon (described in an accompanying paper; doi:10.1038/nature07863). This system minimizes genome modification in induced pluripotent stem cells and enables complete elimination of exogenous reprogramming factors.

    • Keisuke Kaji
    • Katherine Norrby
    • Knut Woltjen
    Research
    Nature
    Volume: 458, P: 771-775

  • Positive selection for gene targeting is a common and reliable method to generate isogenic disease models in human pluripotent stem cells. Here, the authors present engineered selection markers which achieve scarless excision by CRISPR-Cas9 and microhomology mediated end-joining.

    • Shin-Il Kim
    • Tomoko Matsumoto
    • Knut Woltjen
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-14

  • Authentic hypoblast cells created from naive human pluripotent stem cells (hPSCs) spontaneously assemble with naive hPSCs to form a three-dimensional bilaminar structure (bilaminoids) with a pro-amniotic-like cavity.

    • Takumi Okubo
    • Nicolas Rivron
    • Yasuhiro Takashima
    ResearchOpen Access
    Nature
    Volume: 626, P: 357-366

  • How boundary regions form in the epithelia of tissues, for example, the squamous–columnar junction is unclear. Here, the authors identify the transcription factors Sox2 and Gata4 as specifying the transitional epithelium in the murine squamous–columnar junction region in the stomach epithelia.

    • Nao Sankoda
    • Wataru Tanabe
    • Yoshiya Kawaguchi
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-15

  • The scarcity of qualified RNA-binding proteins hinders the development of artificial translational regulators and synthetic gene circuits. Here, the authors repurposed CRISPR-Cas proteins as translational regulators to build synthetic circuits.

    • Shunsuke Kawasaki
    • Hiroki Ono
    • Hirohide Saito
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-17

  • Cell-type diversity results from a series of binary cell fate decisions. Here, Matsuda et al.find that cells engineered with a Notch/Delta lateral inhibition circuit spontaneously bifurcate into Notch-active and Delta-positive subpopulations that are robust at the individual and population levels.

    • Mitsuhiro Matsuda
    • Makito Koga
    • Miki Ebisuya
    Research
    Nature Communications
    Volume: 6, P: 1-12

  • The mechanisms by which in vivo expression of the Yamanaka transcription factors (OSKM) renders somatic cells permissive for differentiation remain unclear. Here, the authors show that in vivo reprogramming using OSKM generates germ cell tumors and drives acquisition of totipotency-like features in somatic cells through DMRT1.

    • Jumpei Taguchi
    • Hirofumi Shibata
    • Yasuhiro Yamada
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-18

  • The EWS-ATF1 fusion gene causes clear cell sarcoma (CCS). Here, the authors show that the downstream effects of EWS-ATF1 expression are strictly context dependent, and reveal the cell of origin for CCS to be Tppp3-expressing cells in peripheral nerves.

    • Shingo Komura
    • Kenji Ito
    • Yasuhiro Yamada
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-19

  • A system involving in vitro induction of presomitic mesoderm recapitulates oscillatory expression of core segmentation clock genes and travelling-wave-like gene expression, suggesting that this system can be used to study the human segmentation clock and provide insights into diseases associated with human axial skeletogenesis.

    • Mitsuhiro Matsuda
    • Yoshihiro Yamanaka
    • Cantas Alev
    Research
    Nature
    Volume: 580, P: 124-129

  • The transcription factor Srf is a central regulator of immediate-early and actin cytoskeletal genes. Here the authors show that Srf is activated by repression of β-actin, promoting iPSC reprogramming of neural progenitor cells and hepatoblasts by repressing cell-type specific genes.

    • Takashi Ikeda
    • Takafusa Hikichi
    • Shinji Masui
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-15

  • Cellular reprogramming and cancer development share properties. Here, the authors examine the impact of in vivo reprogramming on Kras-induced cancer and show reprogramming-mediated repression of somatic cell enhancers in conjunction with Kras mutation results in rapid PDAC development.

    • Hirofumi Shibata
    • Shingo Komura
    • Yasuhiro Yamada
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-16

  • This Review examines how hallmarks of aging manifest in the skin and interact with systemic aging, positioning the skin as both an indicator and driver of age-related physiological decline.

    • David Furman
    • Johan Auwerx
    • Knut Woltjen
    Reviews
    Nature Aging
    Volume: 5, P: 1195-1206

  • This paper uses the piggyBac transposon to generate stable iPS cells from human and mouse fibroblasts; the individual piggyBac insertions can then be removed from established iPS cell lines. The study also demonstrates removal of reprogramming factors joined with 2A sequences (described in an accompanying paper; doi:10.1038/nature07864) delivered by a single transposon from murine iPS lines.

    • Knut Woltjen
    • Iacovos P. Michael
    • Andras Nagy
    Research
    Nature
    Volume: 458, P: 766-770