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Showing 1–20 of 20 results
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  • Light-induced reactions enable three-dimensional objects to be built from simple compounds. Proteins have been added to the list of building blocks, and the resulting gels move in response to environmental cues.

    • April M. Kloxin
    • Kristi S. Anseth
    News & Views
    Nature
    Volume: 454, P: 705-706

  • Advances in photochemistry have profoundly impacted the way in which biology is studied. Now, a photoactivated enzymatic patterning method that offers spatiotemporal control over the presentation of bioactive proteins to direct cells in three-dimensional culture significantly expands the available chemical toolbox.

    • Daniel L. Alge
    • Kristi S. Anseth
    News & Views
    Nature Materials
    Volume: 12, P: 950-952

  • The copper(I)-catalysed azide–alkyne cycloaddition is arguably the most prolific and powerful example of the click reaction paradigm. Here, photochemical reduction of Cu(II) allows spatial and temporal control over the reaction for small-molecule synthesis, patterning of hydrogel formation and the in situ labelling of gels, with features as small as 25 micrometres being produced.

    • Brian J. Adzima
    • Youhua Tao
    • Christopher N. Bowman
    Research
    Nature Chemistry
    Volume: 3, P: 256-259

  • In conditions of prolonged stress valvular cells can contribute to valve disease progression. In this Review, Wang and colleagues describe how the biophysical and biochemical properties of the extracellular matrix can regulate valve cell function in the context of calcific valvular diseases. The authors also describe how new cell culture approaches can be employed to better understand the pathophysiology of valve diseases.

    • Huan Wang
    • Leslie A. Leinwand
    • Kristi S. Anseth
    Reviews
    Nature Reviews Cardiology
    Volume: 11, P: 715-727

  • Fibrosis arises from pathological fibroblast activation into myofibroblasts, causing tissue stiffening. Fibroblast phenotyping is largely subjective, yet crucial for drug screening. Here, authors develop mathematical descriptors of cell morphology and structures to consistently grade fibroblasts.

    • Alex Khang
    • Abigail Barmore
    • Kristi S. Anseth
    ResearchOpen Access
    Nature Communications
    Volume: 16, P: 1-17

  • Cumulative tension on the nuclear membrane of aortic fibroblasts resulting from increases in the stiffness of the extracellular matrix transforms transiently activated fibroblasts into fibrosis-driving myofibroblasts with condensed chromatin.

    • Cierra J. Walker
    • Claudia Crocini
    • Kristi S. Anseth
    Research
    Nature Biomedical Engineering
    Volume: 5, P: 1485-1499

  • The balance of ‘outside–in’ and ‘inside–out’ signaling is critical in tissue development and regeneration. This Comment highlights emerging strategies to engineer and manipulate this delicate equilibrium and fine-tune cellular responses using complementary tools in biomaterials design and synthetic biology.

    • Cole A. DeForest
    • Bruce E. Kirkpatrick
    • Kristi S. Anseth
    Comments & Opinion
    Nature Chemical Engineering
    Volume: 1, P: 2-5

  • Mechanical cues from the local cellular microenvironment can direct cell fate. Now, experiments with human mesenchymal stem cells cultured on phototunable soft poly(ethylene glycol) hydrogels show that the cells remember past physical environments—with the transcriptional co-activators YAP and TAZ acting as a mechanical rheostat—and therefore that appropriate doses of mechanical cues can be used to manipulate the cells’ fate.

    • Chun Yang
    • Mark W. Tibbitt
    • Kristi S. Anseth
    Research
    Nature Materials
    Volume: 13, P: 645-652

  • Cell–matrix interactions have critical roles in regeneration, development and disease. Encapsulated human mesenchymal stem cells can now be induced to differentiate down osteogenic and adipogenic pathways by controlling their three-dimensional environment using tethered small-molecule functional groups.

    • Danielle S. W. Benoit
    • Michael P. Schwartz
    • Kristi S. Anseth
    Research
    Nature Materials
    Volume: 7, P: 816-823

  • Cell-laden synthetic hydrogels — formed via a copper-free click reaction between a poly(ethylene glycol) tetra-cyclooctyne and a peptide-diazide — provide a platform to investigate the cells' response to various stimuli during growth. The hydrogel's biochemical aspects are readily controlled by a thiol-ene photocoupling reaction initiated with visible light, whereas the biomechanical properties of the network are altered via a UV-mediated photodegradation.

    • Cole A. DeForest
    • Kristi S. Anseth
    Research
    Nature Chemistry
    Volume: 3, P: 925-931

  • ‘Click’ chemistry has been broadly exploited, but the intrinsic toxicity of the reactions involved makes its translation to biological applications troublesome. Copper-free click chemistry avoids the problems of toxicity, enabling direct encapsulation of cells within click hydrogels. Tailoring of the gels with biological functionalities is also enabled in real time with micrometre-scale resolution.

    • Cole A. DeForest
    • Brian D. Polizzotti
    • Kristi S. Anseth
    Research
    Nature Materials
    Volume: 8, P: 659-664

  • Organoids recapitulate many aspects of native tissues and even display tissue and organ-level functionality, although with limited control over morphogenesis. This Review describes an emerging framework, termed middle-out tissue engineering, that facilitates spatiotemporal control of tissue-specific cell niches to enable deterministic organoid self-organization and build more advanced in vitro tissue models.

    • Michael R. Blatchley
    • Kristi S. Anseth
    Reviews
    Nature Reviews Bioengineering
    Volume: 1, P: 329-345

  • Tuning the reversible chemistries in hydrogels makes it possible to mimic the dynamic nature of the extracellular matrix. Various chemistries have been incorporated to regulate cell spreading, biochemical presentation and matrix mechanics.

    • Adrianne M. Rosales
    • Kristi S. Anseth
    Reviews
    Nature Reviews Materials
    Volume: 1, P: 1-15

  • Light-based vat-polymerization bioprinting is used to create 3D cell-laden structures via vats filled with photoactivatable bioresins. In this Primer, Levato et al. discuss the experimental design for point-by-point, layer-by-layer and volumetric variations of the technique.

    • Riccardo Levato
    • Oksana Dudaryeva
    • Yu Shrike Zhang
    Reviews
    Nature Reviews Methods Primers
    Volume: 3, P: 1-19