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Showing 1–8 of 8 results
Advanced filters: Author: Kristina Ganzinger Clear advanced filters

  • Single-particle tracking (SPT) has revolutionised studies of protein interactions but is often limited by photobleaching. Here, the authors evolve DNA-PAINT-SPT to enable simultaneous dual-colour detection for the quantification of protein dimerization and live cell membrane protein tracking.

    • Christian Niederauer
    • Chikim Nguyen
    • Kristina A. Ganzinger
    ResearchOpen Access
    Nature Communications
    Volume: 14, P: 1-8

  • The length of single-particle tracking experiments are limited due to photobleaching. Here the authors achieve long-term single-particle tracking with continuous fluorophore exchange in DNA-PAINT and use this to observe DNA origami on lipid bilayers for tens of minutes.

    • Florian Stehr
    • Johannes Stein
    • Petra Schwille
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-8

  • The receptor tyrosine phosphatase CD45 has an important role in T cell activation. Davis and colleagues resolve the structure of CD45 and provide molecular insights into how it contributes to productive T cell receptor triggering.

    • Veronica T Chang
    • Ricardo A Fernandes
    • Simon J Davis
    Research
    Nature Immunology
    Volume: 17, P: 574-582

  • Protein oscillations linked to cell division in Escherichia coli are shown to localize unrelated molecules on the cell membrane via a diffusiophoretic mechanism, in which an effective friction fosters cargo transport along the fluxes set up by the proteins.

    • Beatrice Ramm
    • Andriy Goychuk
    • Petra Schwille
    ResearchOpen Access
    Nature Physics
    Volume: 17, P: 850-858

  • Cilia formation requires Intraflagellar transport (IFT) to move ciliary building blocks and signaling components into the cilium. Here authors use in vitro reconstitution and electron microscopy on IFT172 and reveal its ability to remodel large membrane surfaces into small vesicles.

    • Qianmin Wang
    • Michael Taschner
    • Naoko Mizuno
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-13

  • Genome-wide association studies have established a link between the extracellular chaperone clusterin and susceptibility to Alzheimer's disease. A fluorescence approach is now used to reveal that clusterin sequesters Aβ1–40 oligomers and prevents them from undergoing further aggregation.

    • Priyanka Narayan
    • Angel Orte
    • David Klenerman
    Research
    Nature Structural & Molecular Biology
    Volume: 19, P: 79-83

  • Methylated RNA bases influence many life processes, but current detection methods lack the ability to detect individual methylations in single cells. Here, the authors use fluorescence hybridization probes sensitive to methylation to detect specific epitranscriptomic modifications at the single-cell level.

    • Rohan T. Ranasinghe
    • Martin R. Challand
    • David Klenerman
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-10