Multi-crystal methods such as serial crystallography can provide a complete 3D structure of the target material before radiation damage becomes significant, but the methods are challenging for small molecule crystals with small unit cells, where very few reflections are recorded in a single data image. Here, the authors present a small-rotative fixed-target serial synchrotron crystallography (SR-FT-SSX) methodology, in which rotation of the serial target through a small diffraction angle \((\varphi )\) at each crystal delivers high-quality data, facilitating ab initio unit cell determination and atomic-scale structure solution.
- Sam G. Lewis
- Ben A. Coulson
- Lauren E. Hatcher
