Nature Search

Investigating the analytical robustness of the social and behavioural sciences

When 100 social and behavioural science claims were examined, 34% of reanalyses closely matched the original results, with 74% reaching the same conclusion, revealing limited robustness of single-path analyses and the need to address analytical uncertainty.

Balazs Aczel
Barnabas Szaszi
Brian A. Nosek
Research01 Apr 2026
Nature

Volume: 652, P: 135-142
Decoding ubiquitin signals inside cells

In this Tools of the Trade article, Kiss (Schulman lab) discusses the development of UbiREAD, a method that delivers in vitro-ubiquitinated protein reporters into cells to systematically assess how different ubiquitin chain configurations affect protein stability and degradation.

Leo Kiss
Research Highlights31 Oct 2025
Nature Reviews Molecular Cell Biology

Volume: 26, P: 905
A tri-ionic anchor mechanism drives Ube2N-specific recruitment and K63-chain ubiquitination in TRIM ligases

The E3 ligase TRIM21 targets a broad range of pathogenic proteins using a unique mechanism. Here, the authors combine structural, biochemical and cell biological approaches to elucidate how TRIM21 selectively recruits its canonical E2 partner enzyme Ube2N and catalyzes ubiquitination.

Leo Kiss
Jingwei Zeng
Leo C. James
ResearchOpen Access03 Oct 2019
Nature Communications

Volume: 10, P: 1-13
RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination

The mechanism by which RING E3-anchored ubiquitin chains are formed is not well understood. Here, the authors solve a crystal structure of the RING E3 enzyme TRIM21 trapped in the process of self-anchored chain elongation and provide biochemical and cellular insights into the mechanism of ubiquitin conjugation.

Leo Kiss
Dean Clift
Leo C. James
ResearchOpen Access22 Feb 2021
Nature Communications

Volume: 12, P: 1-12
Trim-Away ubiquitinates and degrades lysine-less and N-terminally acetylated substrates

TRIM21 mediates intracellular antibody immunity and is exploited for targeted protein degradation using Trim-Away technology. Here, the authors dissect the ubiquitination requirements for Trim-Away, providing an explanation for how TRIM21 can target diverse substrates for degradation.

Leo Kiss
Tyler Rhinesmith
Leo C. James
ResearchOpen Access15 Apr 2023
Nature Communications

Volume: 14, P: 1-17
Target-induced clustering activates Trim-Away of pathogens and proteins

The mechanism behind Trim-Away, a protein-depletion approach using E3 ligase, TRIM21 and an antibody against the target, is now clarified, allowing expansion of the toolbox.

Jingwei Zeng
Ana Filipa Santos
Leo C. James
Research25 Feb 2021
Nature Structural & Molecular Biology

Volume: 28, P: 278-289
Mechanistic insights into RNA surveillance by the canonical poly(A) polymerase Pla1 of the MTREC complex

Here the authors show how the MTREC core protein Red1 binds to and sequesters Pla1 from the 3’-end processing machinery to hyperadenylate cryptic unstable transcripts and target them to the exosome for efficient degradation.

Komal Soni
Anusree Sivadas
Tamás Fischer
ResearchOpen Access11 Feb 2023
Nature Communications

Volume: 14, P: 1-20
Plakophilin-2 is required for transcription of genes that control calcium cycling and cardiac rhythm

It is believed that mutations in desmosomal adhesion complex protein plakophilin 2 (PKP2) cause arrhythmia due to loss of cell-cell communication. Here the authors show that PKP2 controls the expression of proteins involved in calcium cycling in adult mouse hearts, and that lack of PKP2 can cause arrhythmia in a structurally normal heart.

Marina Cerrone
Jerome Montnach
Mario Delmar
ResearchOpen Access24 Jul 2017
Nature Communications

Volume: 8, P: 1-16
A Central Small Amino Acid in the VAMP2 Transmembrane Domain Regulates the Fusion Pore in Exocytosis

Benoît Hastoy
Pier A. Scotti
Jochen Lang
ResearchOpen Access06 Jun 2017
Scientific Reports

Volume: 7, P: 1-16

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