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Time-resolved assessment of single-cell protein secretion by sequencing

TRAPS-seq is a sequencing-based method to profile dynamic protein secretion in single cells.

Tongjin Wu
Howard John Womersley
Lih Feng Cheow
Research10 Apr 2023
Nature Methods

Volume: 20, P: 723-734
Identification, sorting and profiling of functional killer cells via the capture of fluorescent target-cell lysate

Subpopulations of functional killer cells in co-culture can be identified and sorted by an assay that detects an intracellular fluorescent protein from lysed cells on the surface of the lysing killer cells.

Yen Hoon Luah
Tongjin Wu
Lih Feng Cheow
Research31 Aug 2023
Nature Biomedical Engineering

Volume: 8, P: 248-262
Single-cell and bulk transcriptome sequencing identifies two epithelial tumor cell states and refines the consensus molecular classification of colorectal cancer

A single-cell transcriptomic analysis of 63 patients with colorectal cancer classifies tumor cells into two epithelial subtypes. An improved tumor classification based on epithelial subtype, microsatellite stability and fibrosis reveals differences in pathway activation and metastasis.

Ignasius Joanito
Pratyaksha Wirapati
Iain Beehuat Tan
ResearchOpen Access30 Jun 2022
Nature Genetics

Volume: 54, P: 963-975
Multiplexed locus-specific analysis of DNA methylation in single cells

Cheow et al. provide a protocol for SCRAM, a method for determining DNA methylation status at defined target sites in single cells. It is reliable, low in cost and relatively fast, making it a good option when full genome coverage is not required.

Lih Feng Cheow
Stephen R Quake
Daniel M Messerschmidt
Protocols26 Mar 2015
Nature Protocols

Volume: 10, P: 619-631
Single-cell multimodal profiling reveals cellular epigenetic heterogeneity

sc-GEM enables the dissection of cellular heterogeneity by simultaneously assaying the status of DNA mutations, gene expression and DNA methylation at multiple targeted loci in individual cells.

Lih Feng Cheow
Elise T Courtois
William F Burkholder
Research15 Aug 2016
Nature Methods

Volume: 13, P: 833-836
Single-cell analyses of human islet cells reveal de-differentiation signatures

Adrian Keong Kee Teo
Chang Siang Lim
Hwee Hui Lau
ResearchOpen Access09 Feb 2018
Cell Death Discovery

Volume: 4, P: 1-11

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Time-resolved assessment of single-cell protein secretion by sequencing

Identification, sorting and profiling of functional killer cells via the capture of fluorescent target-cell lysate

Single-cell and bulk transcriptome sequencing identifies two epithelial tumor cell states and refines the consensus molecular classification of colorectal cancer

Multiplexed locus-specific analysis of DNA methylation in single cells

Single-cell multimodal profiling reveals cellular epigenetic heterogeneity

Single-cell analyses of human islet cells reveal de-differentiation signatures

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