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Showing 1–7 of 7 results

Advanced filters: Author: Louise F. H. Funke Clear advanced filters

Author Correction: Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS

Wesley E. Robertson
Louise F. H. Funke
Jason W. Chin
Amendments and Corrections21 Nov 2024
Nature Protocols

P: 1
Continuous synthesis of E. coli genome sections and Mb-scale human DNA assembly

BAC stepwise insertion synthesis (BASIS) can be used to build synthetic genomes for diverse organisms, and continuous genome synthesis (CGS) enables the rapid synthesis of entire Escherichia coli genomes from functional designs.

Jérôme F. Zürcher
Askar A. Kleefeldt
Jason W. Chin
Research28 Jun 2023
Nature

Volume: 619, P: 555-562
Total synthesis of Escherichia coli with a recoded genome

High-fidelity convergent total synthesis is used to produce Escherichia coli with a 61-codon synthetic genome that uses 59 codons to encode all of the canonical amino acids.

Julius Fredens
Kaihang Wang
Jason W. Chin
Research15 May 2019
Nature

Volume: 569, P: 514-518
Bacterial divisome protein FtsA forms curved antiparallel double filaments when binding to FtsN

Bacterial divisome protein FtsA, which is an actin homologue, forms double filaments following binding to FtsN, and like MreB, an actin homologue in the elongasome, the curvature-sensing double filaments guide peptidoglycan insertion for cell division.

Tim Nierhaus
Stephen H. McLaughlin
Jan Löwe
Research19 Sept 2022
Nature Microbiology

Volume: 7, P: 1686-1701
Rapid discovery and evolution of orthogonal aminoacyl-tRNA synthetase–tRNA pairs

Orthogonal aminoacyl-tRNA synthetase–tRNA pairs are discovered by analyzing millions of sequences.

Daniele Cervettini
Shan Tang
Jason W. Chin
Research13 Apr 2020
Nature Biotechnology

Volume: 38, P: 989-999
Creating custom synthetic genomes in Escherichia coli with REXER and GENESIS

This protocol describes a recombination-based approach for accurate replacement of genomic DNA with large synthetic DNA fragments in E. coli (REXER). Iterative repetition of REXER (GENESIS) combined with genome assembly methods enables the creation of custom synthetic E. coli genomes.

Wesley E. Robertson
Louise F. H. Funke
Jason W. Chin
Protocols26 Apr 2021
Nature Protocols

Volume: 16, P: 2345-2380
The allure of microbiome research: promises of holism and the potential for cruel optimism

Tine Friis
Louise Whiteley
Adam Bencard
ResearchOpen Access16 Apr 2025
Humanities and Social Sciences Communications

Volume: 12, P: 1-13