Stress granules (SGs) are highly dynamic cytoplasmic membraneless organelles that assemble when cells are challenged by stress. Herein, the authors apply a proximity-dependent RNA labeling method, CAP-seq, to comprehensively investigate the content of SG-proximal transcriptome and the dynamic change in SG-proximal transcriptome along the time course of granule assembly and disassembly processes in live mammalian cells.
