Search

After being internalized by cells, nanoparticles can, in rare events, acquire a biomolecular condensate corona. These complexes can reach the extracellular space, where they can deliver their acquired proteins and RNA content to other cells.

Sustainable management of soil organic carbon (SOC) in farmland is critical for mitigating climate change and improving soil health. Degradable film mulching is a promising alternative to plastic film mulching, sequestering SOC and reducing C loss in dryland agroecosystems under climate change.

With whole-genome sequencing of longitudinal Staphylococcus aureus isolates collected from long-term urinary catheters, authors show that the same strain of multidrug resistant S. aureus persists asymptomatically long-term despite antibiotic exposures.

Mineral-associated soil carbon buildup is poorly explained by microbial necromass production (a common hypothesis). During litter decomposition, these processes are decoupled by priming effects and alternate soil carbon formation pathways

In contrast to dsDNA phages where multiple genes are involved in programmed host lysis, ssRNA Fiersviridae phages require only a single gene. Here, genome-wide host suppressors of diverse single-gene lysis systems are identified using a high-throughput genetic screen.

Earthquakes in the lower crust may be facilitated by overpressure of frictional melts, according to pressure estimates from an analysis of quartz inclusions in garnets from pseudotachylytes in the Bergen Arcs.

Biofilm formation and antibiotic tolerance are regulated in Pseudomonas aeruginosa by the protein PA1396, which responds to diffusible signal factors (DSFs) produced by other bacteria. Here, An et al. synthesize DSF analogues that modulate PA1396 activity and thus biofilm formation and antibiotic tolerance.

Lys^M, the lysis protein of the Escherichia coli levivirus M, represents a new ‘protein antibiotic’ that interferes with the synthesis of peptidoglycan by inhibiting lipid II flipping.

The SARS-CoV-2 main protease is an important target for the development of COVID-19 therapeutics. Here, the authors combine X-ray crystallography and mass spectrometry and performed a large scale fragment screening campaign, which yielded 96 liganded structures of this essential viral protein that are of interest for further drug development efforts.

As the emergence of antibiotic resistance has increased, there has been a concurrent decline in the discovery and development of new classes of antibiotics. Fishwick and colleagues explore the possibility that structure-based drug design will plug this hole in the antimicrobial pipeline.

A semiconductor chip, coupled to an ion-sensitive field effect transistor (ISFET) pH sensor, can amplify and quantitate DNA in real time without dyes, cameras and external heating devices.

Leviviruses are phages with ssRNA genomes that encode a protein (Sgl) that induces host autolysis by interfering with bacterial cell wall synthesis. Identification of sgl genes is complicated by their small size and lack of sequence similarity. Here, Chamakura et al. use bioinformatic and experimental approaches to identify sgl genes in 244 leviviral genomes.

By measuring the tidal deformation of Io as it orbits Jupiter using Juno Doppler and historically available data, the hypothesis of a shallow global magma ocean in Io is shown to be false.

FtsN promotes the inward synthesis of septal peptidoglycan (sPG) through the FtsWI complex during bacterial cell division. Here, Lyu et al. apply single-molecule microscopy on E. coli to show that FtsN proteins (I) move processively at a speed similar to that of FtsWI molecules. (II) can be divided into two populations based on their speeds, and (III) their movement is driven exclusively by peptidoglycan synthesis

Juno’s close flyby of Ganymede on 7 June 2021 allowed the infrared mapping spectrometer JIRAM to observe the surface at unprecedented spatial resolution. JIRAM’s detailed spectroscopic characterization reveals past extensive aqueous alteration on the moon, possibly together with hydrothermal activity.

Mycobacterium tuberculosis exhibits complex evolution of antimicrobial resistance (AMR). Here, the authors perform machine learning and structural analysis to identify signatures of AMR evolution to 13 antibiotics.

Most steps in peptidoglycan biosynthesis are understood, but the lipid II binding properties to the flippases MurJ and FtsW remain unknown. Now, it has been shown that lipid II exhibits preferential binding to MurJ, which is modulated by ramoplanin and cardiolipins. These insights into the lipid II binding determinants suggest roles for lipids in regulating substrate binding.

The authors present a public collection of 117 bacterial isolates from the pig gut, including the description of 38 novel taxa. Interesting functions discovered in these organisms include a new fucosyltransferease and sactipeptide-like molecules encoded by biosynthetic gene clusters.

The bacterial pathogen Burkholderia cenocepacia and related species are often multidrug resistant because their cell envelope restricts antibiotic penetration. Here, Hogan et al systematically identify genes associated with resistance and susceptibility to cell envelope-targeting antibiotics, providing insights into underlying mechanisms and suggesting avenues for development of improved antibacterial therapies.

Organisms can adapt to environmental change but how this affects metabolism is unclear. Here, the authors provide a detailed analysis of the effects of individual adaptive mutations on the metabolic network in E. coli, and find evidence of metabolic reprogramming during laboratory evolution on glycerol.

Gain of function methods based on gene overexpression are not easily applied to high-throughput screening across different experimental conditions. Here, the authors present Dub-seq, which separates overexpression library characterization from functional screening and uses random DNA barcodes to increase the experimental throughput.

GpsB is a cytosolic protein that modulates bacterial cell wall synthesis by interacting with cytoplasmic domains of peptidoglycan synthases. Here, Cleverley et al. describe structural features that are important for these interactions, and identify new interacting partners of GpsB in three bacterial species.

BepiColombo mission had already two flybys of Mercury, over the total of six, as planned before entering the planet’s orbit in 2025. Here, the authors show the first ion measurements of Mercury’s inner southern magnetosphere during BepiColombo mission’s first Mercury flyby.

Peptidoglycan is an essential structural component of the cell wall in the majority of bacteria, but the obligate intracellular human pathogen Chlamydia trachomatis was thought to be one of the few exceptions; here a click chemistry approach is used to label peptidoglycan in replicating C. trachomatis with novel d-amino acid dipeptide probes.