Genome editing mediated by zinc finger nucleases can be used to generate fluorescently labelled proteins that are expressed at endogenous levels from their native genetic loci. Applying this technology to the clathrin light chain A and dynamin-2 loci reveals that clathrin-mediated endocytosis is more regular and efficient than previously thought.
- Jeffrey B. Doyon
- Bryan Zeitler
- David G. Drubin
