Nature Search

One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids

CRISPR base editing technologies can be used for disease modelling. Here the authors use various base editing tools to generate tumour models in human adult stem cell-derived hepatocyte, endometrial and intestinal organoids.

Maarten H. Geurts
Shashank Gandhi
Hans Clevers
ResearchOpen Access17 Aug 2023
Nature Communications

Volume: 14, P: 1-18
A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

Rapid identification of host genes essential for virus replication may expedite the generation of therapeutic interventions. Here the authors generate mutant clonal intestinal organoids for 19 host genes previously implicated in coronavirus biology and identify the cell surface protease TMPRSS2 as a potential therapeutic target.

Joep Beumer
Maarten H. Geurts
Hans Clevers
ResearchOpen Access17 Sept 2021
Nature Communications

Volume: 12, P: 1-12
Recapitulating the adenoma–carcinoma sequence by selection of four spontaneous oncogenic mutations in mismatch-repair-deficient human colon organoids

Mizutani et al. use a model that recapitulates the colon adenoma–carcinoma transition through the sequential elimination of media factors leading to spontaneous accumulation of hotspot mutations of mismatch-repair-deficient tumors.

Tomohiro Mizutani
Matteo Boretto
Hans Clevers
ResearchOpen Access01 Nov 2024
Nature Cancer

Volume: 5, P: 1852-1867
Tuft cells act as regenerative stem cells in the human intestine

Four distinct tuft cell states are identified by combining analysis of primary human intestinal resection material and organoids, and studying tuft cell development shows that they represent a damage-induced reserve intestinal stem cell pool in humans.

Lulu Huang
Jochem H. Bernink
Hans Clevers
ResearchOpen Access02 Oct 2024
Nature

Volume: 634, P: 929-935
Uncovering the mode of action of engineered T cells in patient cancer organoids

The dynamics and molecular mechanisms of T cell therapies are probed in cancer organoids.

Johanna F. Dekkers
Maria Alieva
Anne C. Rios
ResearchOpen Access25 Jul 2022
Nature Biotechnology

Volume: 41, P: 60-69
Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis

Organoid models of early liver disease aid target discovery and drug screening.

Delilah Hendriks
Jos F. Brouwers
Hans Clevers
ResearchOpen Access23 Feb 2023
Nature Biotechnology

Volume: 41, P: 1567-1581
Mutational signature in colorectal cancer caused by genotoxic pks⁺ E. coli

Organoids derived from human intestinal cells that are co-cultured with bacteria carrying the genotoxic pks⁺ island develop a distinct mutational signature associated with colorectal cancer.

Cayetano Pleguezuelos-Manzano
Jens Puschhof
Hans Clevers
Research27 Feb 2020
Nature

Volume: 580, P: 269-273
Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

Phage-assisted continuous evolution of Cas9 variants with broad PAM compatibility and high DNA specificity that can be used for transcriptional activation, gene disruption and base editing.

Johnny H. Hu
Shannon M. Miller
David R. Liu
Research28 Feb 2018
Nature

Volume: 556, P: 57-63
CRISPR engineering in organoids for gene repair and disease modelling

Adult stem cell-derived organoids closely resemble their tissue of origin. This Review discusses recent developments in CRISPR-mediated genome engineering and its application using adult-stem-cell-derived organoids in the construction of isogenic disease models and for clinical gene repair.

Maarten H. Geurts
Hans Clevers
Reviews19 Jan 2023
Nature Reviews Bioengineering

Volume: 1, P: 32-45

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One-step generation of tumor models by base editor multiplexing in adult stem cell-derived organoids

A CRISPR/Cas9 genetically engineered organoid biobank reveals essential host factors for coronaviruses

Recapitulating the adenoma–carcinoma sequence by selection of four spontaneous oncogenic mutations in mismatch-repair-deficient human colon organoids

Tuft cells act as regenerative stem cells in the human intestine

Uncovering the mode of action of engineered T cells in patient cancer organoids

Engineered human hepatocyte organoids enable CRISPR-based target discovery and drug screening for steatosis

Mutational signature in colorectal cancer caused by genotoxic pks⁺ E. coli

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

CRISPR engineering in organoids for gene repair and disease modelling

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