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Showing 1–16 of 16 results
Advanced filters: Author: Manu Ben-Johny Clear advanced filters

  • Measuring thein vivo stoichiometry of protein-protein interactions is challenging. Here the authors take a FRET-based approach, quantifying stoichiometry based on ratiometric comparison of donor and acceptor fluorescence, and apply their method to report on a Ca2+-induced switch in calmodulin binding to Ca2+ion channels.

    • Manu Ben-Johny
    • Daniel N. Yue
    • David T. Yue
    ResearchOpen Access
    Nature Communications
    Volume: 7, P: 1-10

  • Skeletal muscle voltage-gated Na+ channel (NaV1.4) activity is subject to calmodulin (CaM) mediated Ca2 +-dependent inactivation while cardiac NaV1.5 is not. Here authors use structural biology, binding and electrophysiology to parse the Ca2 +-dependent changes of CaM when bound to the NaV1.4.

    • Jesse B. Yoder
    • Manu Ben-Johny
    • L. Mario Amzel
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-12

  • This protocol describes a nondestructive FRET-based assay for measuring protein interactions in cultured cells. The correlation between FRET efficiencies and relative protein concentration can be used to determine relative binding affinities.

    • Elisabeth S Butz
    • Manu Ben-Johny
    • Christian Wahl-Schott
    Protocols
    Nature Protocols
    Volume: 11, P: 2470-2498

  • Ranolazine is an anti-arrhythmic drug that targets voltage-gated sodium channels. Lenaeus et al. present a cryo-EM structure of the cardiac sodium channel bound to ranolazine to demonstrate that this compound acts as a structural clamp that inhibits the channels.

    • Lucile Fossier
    • Manu Ben-Johny
    News & Views
    Nature Cardiovascular Research
    Volume: 2, P: 494-495

  • Papa et al. show that phosphorylation by PKA of four residues in Rad, a calcium channel inhibitor, is required to mediate the β-adrenergic-induced increase in calcium current and contractile force. Additionally, Rad-phosphosite-mutant mice showed reduced basal heart rate and contractility. Conversely, expression of mutant calcium channel unable to bind wild-type or phosphosite-mutant Rad was sufficient to enhance basal calcium influx and contractility, independently of β-adrenergic stimulation.

    • Arianne Papa
    • Sergey I. Zakharov
    • Steven O. Marx
    ResearchOpen Access
    Nature Cardiovascular Research
    Volume: 1, P: 1022-1038

  • Increased late sodium current due to the disruption of voltage-gated sodium channel inactivation is a common pathogenic mechanism in different arrhythmogenic syndromes. Chakouri et al. show that fibroblast growth factor homologous factors (FHFs) modulate pathogenic late sodium current in an isoform-specific way, and leverage this observation to design and test a prototype of inhibitor peptide based on a protein domain from an inhibitory FHF isoform.

    • Nourdine Chakouri
    • Sharen Rivas
    • Manu Ben-Johny
    Research
    Nature Cardiovascular Research
    Volume: 1, P: 1-13

  • An in vivo approach to identify proteins whose enrichment near cardiac CaV1.2 channels changes upon β-adrenergic stimulation finds the G protein Rad, which is phosphorylated by protein kinase A, thereby relieving channel inhibition by Rad and causing an increased Ca2+ current.

    • Guoxia Liu
    • Arianne Papa
    • Steven O. Marx
    Research
    Nature
    Volume: 577, P: 695-700

  • Auxiliary subunit molecular diversity is an unexploited target for developing ion channel inhibitors. Here, the authors develop a general approach to inhibit ion channels based on the resident auxiliary subunit isoform to elucidate unique functions of distinct ion channel macromolecular complexes.

    • Travis J. Morgenstern
    • Neha Nirwan
    • Henry M. Colecraft
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-19

  • A tethered ligand approach reveals that calcium-binding proteins (CaBPs) act as allosteric modulators of calcium channel calmodulin regulation, shedding light on how trace CaBPs can prevail over an abundance of CaM.

    • Philemon S Yang
    • Manu Ben Johny
    • David T Yue
    Research
    Nature Chemical Biology
    Volume: 10, P: 231-238

  • Manning Zhang et al. investigate how the mouse brain makes sense of multiple sensory types, such as sound and touch using two-photon Ca2+ imaging in the somatosensory cortex while exposing the mouse to sound and whisker simulation. They identify a potential mutually-inhibitory circuit between sound and touch that depends on the relative frequencies of the different stimuli.

    • Manning Zhang
    • Sung Eun Kwon
    • John B. Issa
    ResearchOpen Access
    Communications Biology
    Volume: 3, P: 1-17