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Showing 1–2 of 2 results
Advanced filters: Author: Marco J. Lobba Clear advanced filters

  • Doudna and colleagues determine the mechanisms used by type V anti-CRISPR proteins. AcrVA1 is a multiple-turnover inhibitor that triggers cleavage of the Cas12a-bound guide RNA, while AcrVA4 and AcrVA5 inhibit recognition of dsDNA.

    • Gavin J. Knott
    • Brittney W. Thornton
    • Jennifer A. Doudna
    Research
    Nature Structural & Molecular Biology
    Volume: 26, P: 315-321

  • Self-assembling proteins have important applications in industrial technologies, but it is difficult to predict how mutations affect assembly. Here the authors present SyMAPS, coupling comprehensive codon mutagenesis with high-throughput sequencing, and apply it to bacteriophage capsid protein.

    • Emily C. Hartman
    • Christopher M. Jakobson
    • Danielle Tullman-Ercek
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-11