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Showing 1–4 of 4 results
Advanced filters: Author: Maria Harkiolaki Clear advanced filters

  • In this study, Peijun Zhang and colleagues use cryoFIB/SEM volume imaging and soft x-ray cryo-tomography with cryo-electron tomography (cryoET) of cellular periphery, lamellae, and subtomogram averaging to place critical structural events in the SARS-CoV-2 infection cycle in the context of whole-cell images.

    • Luiza Mendonça
    • Andrew Howe
    • Peijun Zhang
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-10

  • This protocol describes sample-preparation strategies for correlative 3D cryo-structured illumination microscopy and cryo-soft X-ray tomography. The authors also provide a direct comparison and recommendations regarding the selection and use of fiducials for 3D correlation.

    • Chidinma A. Okolo
    • Ilias Kounatidis
    • Maria Harkiolaki
    Protocols
    Nature Protocols
    Volume: 16, P: 2851-2885

  • Bioimaging data have significant potential for reuse, but unlocking this potential requires systematic archiving of data and metadata in public databases. We propose draft metadata guidelines to begin addressing the needs of diverse communities within light and electron microscopy. We hope this publication and the proposed Recommended Metadata for Biological Images (REMBI) will stimulate discussions about their implementation and future extension.

    • Ugis Sarkans
    • Wah Chiu
    • Alvis Brazma
    Comments & Opinion
    Nature Methods
    Volume: 18, P: 1418-1422