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Showing 1–3 of 3 results
Advanced filters: Author: Mario Mietzsch Clear advanced filters

  • Single-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an adeno-associated virus serotype 2 variant.

    • Yong Zi Tan
    • Sriram Aiyer
    • Dmitry Lyumkis
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-11

  • Here, the authors quantify the effect of cryo-EM data acquisition with stage-tilt on the global resolution of reconstructions and present a tool for predicting an optimal stage-tilt angle to ameliorate the effects of preferred specimen orientation.

    • Sriram Aiyer
    • Philip R. Baldwin
    • Dmitry Lyumkis
    ResearchOpen Access
    Nature Communications
    Volume: 15, P: 1-19