The α-oxoamine synthases (AOSes) are a class of pyridoxal 5’-phosphate-dependent enzymes that catalyse Claisen-like condensation of amino acids with an acyl-CoA thioester to generate α-aminoketones, however, their application in chemoenzymatic synthesis remains limited due to the substrate specificity. Here, the authors report the structure-guided engineering of thermostable AOS from Thermus thermophilus to expand the range of amino acids and simplify N-acetylcysteamine acyl-thioester substrates.
- Ben Ashley
- Sam Mathew
- Dominic J. Campopiano
