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Showing 1–2 of 2 results
Advanced filters: Author: Markus Kilisch Clear advanced filters
  • Epitope tags are widely used in various applications, but often lack versatility. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be used in a range of different applications, from protein purification to super-resolution imaging and in vivo detection of proteins.

    • Hansjörg Götzke
    • Markus Kilisch
    • Steffen Frey
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-12
  • The TOM and TIM23 complexes facilitate the transport of nuclear-encoded proteins into the mitochondrial matrix. Here, the authors use a stalled client protein to purify the translocation supercomplex and gain insight into the TOM-TIM23 interface and the mechanism of protein handover from the TOM to the TIM23 complex.

    • Ridhima Gomkale
    • Andreas Linden
    • Peter Rehling
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-17