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A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

Currently, genetically encoded calcium indicators are not suitable for direct quantification. Here the authors engineer a fluorescence lifetime imaging calcium biosensor, Turquoise Calcium Fluorescence LIfeTime Sensor (Tq-Ca-FLITS), and measure intracellular calcium concentrations in human-derived organoids.

Franka H. van der Linden
Eike K. Mahlandt
Joachim Goedhart
ResearchOpen Access09 Dec 2021
Nature Communications

Volume: 12, P: 1-13
Genetic landscape of chronic obstructive pulmonary disease identifies heterogeneous cell-type and phenotype associations

Genome-wide analysis of chronic obstructive pulmonary disease identifies 82 loci, 35 of which are new. Integration of gene expression and genomic annotation data shows enrichment of signals in lung tissue, smooth muscle and several lung cell types.

Phuwanat Sakornsakolpat
Dmitry Prokopenko
Loems Zeigler-Heitbrock
Research25 Feb 2019
Nature Genetics

Volume: 51, P: 494-505
In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots

Imaging in living Arabidopsis roots reveals that protein complexes can change their conformation in a cell-type-dependent manner to regulate specific gene expression programs leading to precise specification and maintenance of particular cell fates within the root meristem.

Yuchen Long
Yvonne Stahl
Ikram Blilou
Research26 Jul 2017
Nature

Volume: 548, P: 97-102
mScarlet: a bright monomeric red fluorescent protein for cellular imaging

An extremely bright, truly monomeric RFP, mScarlet, is described that outperforms existing RFPs in diverse labeling applications, especially in FRET with ratiometric imaging.

Daphne S Bindels
Lindsay Haarbosch
Theodorus W J Gadella Jr
Research21 Nov 2016
Nature Methods

Volume: 14, P: 53-56
Domain analysis of the Nematostella vectensis SNAIL ortholog reveals unique nucleolar localization that depends on the zinc-finger domains

Ada A. Dattoli
Mark A. Hink
Marten Postma
ResearchOpen Access20 Jul 2015
Scientific Reports

Volume: 5, P: 1-20
The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation

Eelco Hoogendoorn
Kevin C. Crosby
Marten Postma
ResearchOpen Access24 Jan 2014
Scientific Reports

Volume: 4, P: 1-10
Multiparameter screening method for developing optimized red-fluorescent proteins

This protocol describes a screening pipeline to select red fluorescent proteins (FPs) with optimized parameters from a mutant FP library. FPs are screened for fluorescence lifetime, cellular brightness, maturation efficiency, and photostability.

Daphne S. Bindels
Marten Postma
Theodorus W. J. Gadella Jr
Protocols15 Jan 2020
Nature Protocols

Volume: 15, P: 450-478
Temporal tracking of quantum-dot apatite across in vitro mycorrhizal networks shows how host demand can influence fungal nutrient transfer strategies

Anouk van’t Padje
Loreto Oyarte Galvez
E. Toby Kiers
ResearchOpen Access28 Sept 2020
The ISME Journal

Volume: 15, P: 435-449
Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization

Jakobus van Unen
Nathalie R. Reinhard
Joachim Goedhart
ResearchOpen Access05 Oct 2015
Scientific Reports

Volume: 5, P: 1-16
Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

Marieke Mastop
Daphne S. Bindels
Joachim Goedhart
ResearchOpen Access20 Sept 2017
Scientific Reports

Volume: 7, P: 1-18

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A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

Genetic landscape of chronic obstructive pulmonary disease identifies heterogeneous cell-type and phenotype associations

In vivo FRET–FLIM reveals cell-type-specific protein interactions in Arabidopsis roots

mScarlet: a bright monomeric red fluorescent protein for cellular imaging

Domain analysis of the Nematostella vectensis SNAIL ortholog reveals unique nucleolar localization that depends on the zinc-finger domains

The fidelity of stochastic single-molecule super-resolution reconstructions critically depends upon robust background estimation

Multiparameter screening method for developing optimized red-fluorescent proteins

Temporal tracking of quantum-dot apatite across in vitro mycorrhizal networks shows how host demand can influence fungal nutrient transfer strategies

Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization

Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

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