Crosslinking mass spectrometry (XL-MS) has become a crucial tool for studying protein structures and interactions in their native environments, however, the current cleavable linkers are fraught with practical limitations. Here, the authors synthesize and characterize DiSPASO, a novel lysine-reactive, MS-cleavable, and membrane-permeable crosslinker with an alkyne-based click chemistry handle for affinity enrichment, showing potential for improving protein-protein interaction mapping.
- Fränze Müller
- Bogdan R. Brutiu
- Karl Mechtler
