Extracellular vesicles are being explored for various biomedical applications, but face challenges with regard to isolation, characterization and manufacturing. This Review delineates the physico-chemical landscape of extracellular vesicles to identify the distinct challenges associated with the manufacturing and isolation of extracellular vesicles from biofluids and suggests opportunities to address them.
- Mauro Manno
- Antonella Bongiovanni
- Paolo Arosio
