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Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate

Incorporation of mismatched nucleotides during DNA replication or repair can lead to mutagenesis. Here the authors reveal that DNA ligase can ligate NHEJ intermediates following incorporation of 8-oxodGTP or dGTP opposite T by DNA Polymerase mu (Pol mu) in vitro, which suggests that Pol mu could cause promutagenic mismatches during DSB repair.

Melike Çağlayan
Samuel H. Wilson
ResearchOpen Access11 Oct 2018
Nature Communications

Volume: 9, P: 1-4
Oxidized nucleotide insertion by pol β confounds ligation during base excision repair

Oxidative stress in cells leads to the oxidations of DNA precursors. Here the authors show that these oxidized precursors can be incorporatedin vivoduring base excision repair, leading to DNA breaks and cytotoxicity.

Melike Çağlayan
Julie K. Horton
Samuel H. Wilson
ResearchOpen Access09 Jan 2017
Nature Communications

Volume: 8, P: 1-11
Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair

5'-adenylated DNA adducts generated during nucleotide excision repair (NER) are removed by aprataxin to permit DNA end ligation. Now, structural and kinetic analyses reveal that NER enzymes DNA polymerase β and FEN1 can also excise these adducts and thus provide a 'backup' repair pathway for abasic sites.

Melike Çağlayan
Vinod K Batra
Samuel H Wilson
Research28 Apr 2014
Nature Structural & Molecular Biology

Volume: 21, P: 497-499
Structures of LIG1 that engage with mutagenic mismatches inserted by polβ in base excision repair

Ligase I seals two ends of DNA during DNA repair and replication. Here — solving structures of Ligase I with imperfect DNA harboring mismatches incorporated by DNA polymerase — the authors reveal how the repair proteins maintain fidelity at final ligation step.

Qun Tang
Mitchell Gulkis
Melike Çağlayan
ResearchOpen Access05 Jul 2022
Nature Communications

Volume: 13, P: 1-11
Pol μ ribonucleotide insertion opposite 8-oxodG facilitates the ligation of premutagenic DNA repair intermediate

Melike Çağlayan
ResearchOpen Access22 Jan 2020
Scientific Reports

Volume: 10, P: 1-14

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Pol μ dGTP mismatch insertion opposite T coupled with ligation reveals promutagenic DNA repair intermediate

Oxidized nucleotide insertion by pol β confounds ligation during base excision repair

Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair

Structures of LIG1 that engage with mutagenic mismatches inserted by polβ in base excision repair

Pol μ ribonucleotide insertion opposite 8-oxodG facilitates the ligation of premutagenic DNA repair intermediate

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