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Showing 1–7 of 7 results
Advanced filters: Author: Michael A. Cianfrocco Clear advanced filters

  • Recent advances in cryo-electron microscopy are enabling researchers to solve protein structures at near-atomic resolutions, expanding the biological applicability of this technique. Michael Eisenstein reports.

    • Michael Eisenstein
    News
    Nature Methods
    Volume: 13, P: 19-22

  • MLL family histone methyltransferases deposit histone H3 Lys4 mono-/di-/tri-methylation and regulate gene expression in mammals. Here the authors report the single-particle cryo-EM structure of the NCP-bound human MLL1 core complex, shedding light on how the MLL1 complex engages chromatin and how chromatin binding promotes MLL1 tri-methylation activity.

    • Sang Ho Park
    • Alex Ayoub
    • Uhn-Soo Cho
    ResearchOpen Access
    Nature Communications
    Volume: 10, P: 1-13

  • Human Dicer can process long double-stranded RNA and hairpin precursor RNA to yield short interfering RNAs or microRNAs, respectively. EM and single-particle analyses of Dicer–substrate complexes now provide insight into the structural basis of Dicer's substrate preference, implicating RNA structure and cofactors in determining substrate recognition and processing efficiency by Dicer.

    • David W Taylor
    • Enbo Ma
    • Hong-Wei Wang
    Research
    Nature Structural & Molecular Biology
    Volume: 20, P: 662-670

  • In eukaryotes, DNA replication depends on loading the ring-shaped helicase, Mcm2–7 onto double-stranded DNA and subsequent activation by GINS–Cdc45. Using single-particle EM reconstructions, the Mcm2–7 forms ring hexamers that are open between Mcm2 and Mcm5. When present, GINS and Cdc45 both seal off this gap. In the presence of a non-hydrolyzable ATP analog, two pores are formed. In this way, the complex could promote duplex opening and then segregate the two strands by partitioning them into the two pores.

    • Alessandro Costa
    • Ivar Ilves
    • James M Berger
    Research
    Nature Structural & Molecular Biology
    Volume: 18, P: 471-477