Nature Search

Extending the recognition site of designed minor groove binding molecules

A linked, imazole/pyrrole minor-groove ligand has been shown to bind sequence specif icially to a 13 base-pair target sequence in a mixed 1:1/2:1 mode.

Bernhard H. Geierstanger
Milan Mrksich
David E. Wemmer
Correspondence01 Apr 1996
Nature Structural Biology

Volume: 3, P: 321-324
Peptide chips for the quantitative evaluation of protein kinase activity

Benjamin T. Houseman
Joon H. Huh
Milan Mrksich
Research01 Mar 2002
Nature Biotechnology

Volume: 20, P: 270-274
Label-assisted mass spectrometry for the acceleration of reaction discovery and optimization

The development of new reaction methodology is a core activity for organic chemists, but the process can be slow and can rely on serendipity. Here, a reaction discovery platform is described that relies on the labelling of a reaction component with a chemical tag that enables rapid mass spectrometric detection of the derived products in complex mixtures.

Jaime R. Cabrera-Pardo
David I. Chai
Sergey A. Kozmin
Research31 Mar 2013
Nature Chemistry

Volume: 5, P: 423-427
The emerging era of structural nanomedicine

The lack of structural definition in nanomedicines limits therapeutic efficacy and complicates regulatory approval. Here, we emphasize that defining, designing and optimizing the structures of nanomedicines are critical to developing effective therapies because their architectures — not just the identity of their components — determines potency.

Chad A. Mirkin
Milan Mrksich
Natalie Artzi
Comments & Opinion25 Apr 2025
Nature Reviews Bioengineering

Volume: 3, P: 526-528
Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Thapakorn Jaroentomeechai
Jessica C. Stark
Matthew P. DeLisa
Amendments and CorrectionsOpen Access20 Aug 2018
Nature Communications

Volume: 9, P: 1
Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

The GlycoSCORES method, which involves cell-free protein expression and substrate-site profiling of glycosyltransferase enzymes by SAMDI–MS, enables the identification of glycosylation tags for glycoengineering efforts.

Weston Kightlinger
Liang Lin
Michael C. Jewett
Research07 May 2018
Nature Chemical Biology

Volume: 14, P: 627-635
Exploration of the nanomedicine-design space with high-throughput screening and machine learning

A methodology combining high-throughput nanoparticle synthesis and machine learning can be used to efficiently explore structure–activity relationships for nanomedicines, as shown by spherical nucleic acids functioning as cancer-vaccine candidates.

Gokay Yamankurt
Eric J. Berns
Chad A. Mirkin
Research18 Feb 2019
Nature Biomedical Engineering

Volume: 3, P: 318-327
Discovery of glycosyltransferases using carbohydrate arrays and mass spectrometry

Discovery of the native activity of the ~60,000 putative glycosyltransferases remains a substantial challenge. A high-throughput, label-free method drastically speeds this process, with assays of 85 enzymes, 24 acceptors and 7 donors returning functions for four new proteins.

Lan Ban
Nicholas Pettit
Milan Mrksich
Research22 Jul 2012
Nature Chemical Biology

Volume: 8, P: 769-773
Computational planning of the synthesis of complex natural products

A synthetic route-planning algorithm, augmented with causal relationships that allow it to strategize over multiple steps, can design complex natural-product syntheses that are indistinguishable from those designed by human experts.

Barbara Mikulak-Klucznik
Patrycja Gołębiowska
Bartosz A. Grzybowski
Research13 Oct 2020
Nature

Volume: 588, P: 83-88
Multipotency retained

Stem cells that are cultured in the laboratory differentiate in response to the mechanical properties of the substrate and its topography. It is now shown that mesenchymal stem cell multipotency is prolonged when the cells are cultured on a surface patterned with an ordered arrangement of nanoscale pits.

Milan Mrksich
News & Views22 Jul 2011
Nature Materials

Volume: 10, P: 559-560
Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria

An attractive route for carbon-negative synthesis of biochemical products is the reverse β-oxidation pathway coupled to the Wood-Ljungdahl pathway. Here the authors use a high-throughput in vitro prototyping workflow to screen 762 unique pathway combinations using cell-free extracts tailored for r-BOX to identify enzyme sets for enhanced product selectivity.

Bastian Vögeli
Luca Schulz
Michael C. Jewett
ResearchOpen Access01 Jun 2022
Nature Communications

Volume: 13, P: 1-10
A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

Constructing biosynthetic pathways to study and engineer glycoprotein structures is difficult. Here, the authors use GlycoPRIME, a cell-free workflow for mixing-and-matching glycosylation enzymes, to evaluate 37 putative glycosylation pathways and discover routes to 18 new glycoprotein structures

Weston Kightlinger
Katherine E. Duncker
Michael C. Jewett
ResearchOpen Access27 Nov 2019
Nature Communications

Volume: 10, P: 1-13
NADH inhibition of SIRT1 links energy state to transcription during time-restricted feeding

Using a model of time-restricted feeding in mice, Levine et al. show that the hepatic NADH cycle links nutrient state to whole-body energetics through the rhythmic regulation of SIRT1.

Daniel C. Levine
Hsin-Yu Kuo
Joseph Bass
ResearchOpen Access13 Dec 2021
Nature Metabolism

Volume: 3, P: 1621-1632
Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

The ability to produce homogeneous glycoproteins is expected to advance fundamental understanding in glycoscience, but current in vivo-based production systems have several limitations. Here, the authors develop an E. coli extract-based one-pot system for customized production of N-linked glycoproteins.

Thapakorn Jaroentomeechai
Jessica C. Stark
Matthew P. DeLisa
ResearchOpen Access12 Jul 2018
Nature Communications

Volume: 9, P: 1-11
Chemical screening by mass spectrometry to identify inhibitors of anthrax lethal factor

Dal-Hee Min
Wei-Jen Tang
Milan Mrksich
Research16 May 2004
Nature Biotechnology

Volume: 22, P: 717-723
Three-component reaction discovery enabled by mass spectrometry of self-assembled monolayers

A general reaction-discovery platform has been used for identification of a new multicomponent transformation. The approach entails rapid analysis of interfacial chemical reactions on arrays of self-assembled monolayers using mass spectrometry. This enabled identification of a simple organic phosphine that catalyses a previously unknown condensation of siloxy alkynes, aldehydes and amines.

Timothy J. Montavon
Jing Li
Sergey A. Kozmin
Research04 Dec 2011
Nature Chemistry

Volume: 4, P: 45-51
Surface potential modulation as a tool for mitigating challenges in SERS-based microneedle sensors

Vitor Brasiliense
Ji Eun Park
Milan Mrksich
ResearchOpen Access23 Sept 2022
Scientific Reports

Volume: 12, P: 1-9
Tyrosine phosphatase activity is restricted by basic charge substituting mutation of substrates

Che-Fan Huang
Cara J. Gottardi
Milan Mrksich
ResearchOpen Access05 Sept 2022
Scientific Reports

Volume: 12, P: 1-9
Morphological features of single cells enable accurate automated classification of cancer from non-cancer cell lines

Zeynab Mousavikhamene
Daniel J. Sykora
Neda Bagheri
ResearchOpen Access21 Dec 2021
Scientific Reports

Volume: 11, P: 1-11
How many human proteoforms are there?

Ruedi Aebersold
Jeffrey N Agar
Bing Zhang
Reviews14 Feb 2018
Nature Chemical Biology

Volume: 14, P: 206-214

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