Sinclair et al. combine quantitative proteomics and an autophagy flux reporter to map autophagy substrates and triggers during CD8 T cell differentiation. Proteins degraded and fueled by autophagy in naïve and effector T cells are identified and it is revealed that the regulation of amino acid transport allows antigen receptors and inflammatory cytokines to repress autophagy flux to shape T cell differentiation.
- Linda V. Sinclair
- Tom Youdale
- Doreen A. Cantrell
