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Showing 1–8 of 8 results
Advanced filters: Author: Miroslava Schaffer Clear advanced filters

  • While the architecture of vertebrate nuclear pore complexes (NPCs) is well understood, the extent of its evolutionary conservation is still unclear. Here, the authors analyze the in situ architecture of an algal NPC, revealing distinct structural features that provide insights into NPC evolution.

    • Shyamal Mosalaganti
    • Jan Kosinski
    • Martin Beck
    ResearchOpen Access
    Nature Communications
    Volume: 9, P: 1-8

  • The translocon-associated protein complex (TRAP) is a crucial component of the endoplasmic reticulum protein translocon. Here the authors study native translocon structures from human disease patients and algae cells to determine the molecular organization of the TRAP complex.

    • Stefan Pfeffer
    • Johanna Dudek
    • Friedrich Förster
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-9

  • Several unusual features have been reported for bacteria of the phylum Planctomycetes, such as cytosolic compartmentalization and an endocytosis-like process. Here, Boedekeret al. provide evidence supporting a Gram-negative cell plan and the absence of endocytosis-like processes in these organisms.

    • Christian Boedeker
    • Margarete Schüler
    • Christian Jogler
    ResearchOpen Access
    Nature Communications
    Volume: 8, P: 1-14

  • High-resolution structural analysis of macromolecular complexes by cryo-ET requires extremely thin samples. This protocol describes how to prepare thin specimens using FIB milling from frozen cells on grids, which enables direct structural analysis of biomolecules in their native environments, i.e., cells.

    • Felix R. Wagner
    • Reika Watanabe
    • Elizabeth Villa
    Protocols
    Nature Protocols
    Volume: 15, P: 2041-2070