Polyelectrolyte multilayers-modified membrane filter, leading to achieve rapid immunoassay as compared with the conventional enzyme-linked immunosorbent assay (ELISA) system. The antigen–antibody reaction was carried out by the centrifugal permeation of antigen solution allowing the local condensation of the antigen molecules in the proximity of the primary antibody. Hence, the incubation time for the antigen–antibody reaction was just permeation time, 3 min, which overcomes the molecular diffusion as rate-limiting factor compared with conventional ELISA method.
- Heyun Shen
- Junji Watanabe
- Mitsuru Akashi
