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Showing 1–3 of 3 results
Advanced filters: Author: Momcilo Gavrilov Clear advanced filters

  • PCR is an essential method for the amplification and manipulation of nucleic acids, but the requirement for a thermocycler limits access. Here, authors engineer a helicase to replace the heating step of PCR with enzymatic unwinding, allowing the isothermal amplification of fragments up to 6 kb.

    • Momčilo Gavrilov
    • Joshua Y. C. Yang
    • Taekjip Ha
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-14

  • Visualisation of point mutations in situ is informative for studying genetic diseases. Here the authors report single guide genome oligopaint via local denaturation fluorescence in situ hybridisation, sgGOLDFISH, a direct hybridisation genome imaging method with single-nucleotide sensitivity.

    • Yanbo Wang
    • W. Taylor Cottle
    • Taekjip Ha
    ResearchOpen Access
    Nature Communications
    Volume: 13, P: 1-12

  • A new method, SENTINEL, improves the specificity of nucleic acid target sequence identification via a combination of isothermal amplification and sequence-specific DNA endonucleases. The method can discriminate single base-pair differences and takes less than 1 h to complete.

    • Roger S. Zou
    • Momcilo Gavrilov
    • Taekjip Ha
    ResearchOpen Access
    Communications Biology
    Volume: 5, P: 1-9