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The design, manufacture and LNP formulation of mRNA for research use

This protocol describes simple, small-scale laboratory production of mRNA for research and preclinical use. This includes sequence design, DNA template production, mRNA synthesis by in vitro transcription, formulation into lipid nanoparticles and transfection into cultured cells.

Laura J. Leighton
Natasha Chaudhary
Tim R. Mercer
Protocols10 Jun 2025
Nature Protocols

Volume: 20, P: 3552-3581
Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

Caveolae are spherical nanodomains of the plasma membrane generated by assembly of caveolin and cavin proteins. Here, the authors show that fuzzy electrostatic interactions between caveolin-1 and Cavin1 proteins, combined with membrane lipid interactions, are required to generate membrane curvature and a metastable caveola coat.

Vikas A. Tillu
James Rae
Brett M. Collins
ResearchOpen Access10 Feb 2021
Nature Communications

Volume: 12, P: 1-18
CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition

Genome-scale CRISPR–Cas9-based synthetic lethality screens identify PKMYT1 as a potential therapeutic target in tumours with CCNE1 amplification.

David Gallo
Jordan T. F. Young
Daniel Durocher
ResearchOpen Access20 Apr 2022
Nature

Volume: 604, P: 749-756
The CIP2A–TOPBP1 axis safeguards chromosome stability and is a synthetic lethal target for BRCA-mutated cancer

Durocher and colleagues identify CIP2A through synthetic lethal CRISPR screens as a key regulator of adaptive feedback mechanisms controlling chromosomal stability arising from accumulated DNA lesions in BRCA-mutated tumor cells.

Salomé Adam
Silvia Emma Rossi
Daniel Durocher
Research11 Nov 2021
Nature Cancer

Volume: 2, P: 1357-1371
Mapping the human genetic architecture of COVID-19

A global network of researchers was formed to investigate the role of human genetics in SARS-CoV-2 infection and COVID-19 severity; this paper reports 13 genome-wide significant loci and potentially actionable mechanisms in response to infection.

Mari E. K. Niemi
Juha Karjalainen
Chloe Donohue
ResearchOpen Access08 Jul 2021
Nature

Volume: 600, P: 472-477
Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells

Plasma membrane tension is an important factor that regulates many key cellular processes. Here authors show that a specific dynamin-independent endocytic pathway is modulated by changes in tension via the mechano-transducer vinculin.

Joseph Jose Thottacherry
Anita Joanna Kosmalska
Satyajit Mayor
ResearchOpen Access11 Oct 2018
Nature Communications

Volume: 9, P: 1-14
Whole-genome sequencing reveals host factors underlying critical COVID-19

Whole-genome sequencing, transcriptome-wide association and fine-mapping analyses in over 7,000 individuals with critical COVID-19 are used to identify 16 independent variants that are associated with severe illness in COVID-19.

Athanasios Kousathanas
Erola Pairo-Castineira
J. Kenneth Baillie
ResearchOpen Access07 Mar 2022
Nature

Volume: 607, P: 97-103
Antibody decay, T cell immunity and breakthrough infections following two SARS-CoV-2 vaccine doses in inflammatory bowel disease patients treated with infliximab and vedolizumab

Vaccination is effective in protecting from COVID-19. Here the authors report immune responses and breakthrough infections in twice-vaccinated patients receiving anti-TNF treatments for inflammatory bowel disease, and find dampened vaccine responses that implicate the need of adapted vaccination schedules for these patients.

Simeng Lin
Nicholas A. Kennedy
Jeannie Bishop
ResearchOpen Access16 Mar 2022
Nature Communications

Volume: 13, P: 1-14
Regulation of the divalent metal ion transporter via membrane budding

KimberlyD Mackenzie
Natalie J Foot
Sharad Kumar
ResearchOpen Access21 Jun 2016
Cell Discovery

Volume: 2, P: 1-14

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The design, manufacture and LNP formulation of mRNA for research use

Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

CCNE1 amplification is synthetic lethal with PKMYT1 kinase inhibition

The CIP2A–TOPBP1 axis safeguards chromosome stability and is a synthetic lethal target for BRCA-mutated cancer

Mapping the human genetic architecture of COVID-19

Mechanochemical feedback control of dynamin independent endocytosis modulates membrane tension in adherent cells

Whole-genome sequencing reveals host factors underlying critical COVID-19

Antibody decay, T cell immunity and breakthrough infections following two SARS-CoV-2 vaccine doses in inflammatory bowel disease patients treated with infliximab and vedolizumab

Regulation of the divalent metal ion transporter via membrane budding

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