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Advanced filters: Author: Nelly Helmbrecht Clear advanced filters

Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Some DNA sequences are refractory to CRISPR-Cas9 cleavage, partially due to gRNA misfolding. Here the authors engineer gRNAs to prevent misfolding and further enhanced their stability by chemical modifications allowing robust genome editing regardless of target sequence.

Stephan Riesenberg
Nelly Helmbrecht
Svante Pääbo
ResearchOpen Access25 Jan 2022
Nature Communications

Volume: 13, P: 1-8
Efficient high-precision homology-directed repair-dependent genome editing by HDRobust

HDRobust is a high-precision tool for homology-directed repair-mediated single-nucleotide editing.

Stephan Riesenberg
Philipp Kanis
Svante Pääbo
ResearchOpen Access20 Jul 2023
Nature Methods

Volume: 20, P: 1388-1399

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Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Efficient high-precision homology-directed repair-dependent genome editing by HDRobust

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